Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gentamicin antigen. Standards or samples are added to the appropriate microtiter plate wells with Gentamicin specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Gentamicin and Gentamicin in standards or samples with the Gentamicin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Gentamicin in the standards or samples. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Gentamicin (GEN) belongs to aminoglycoside antibiotics, playing the main role in gram-negative bacteria. It is widely used in veterinary clinical and animal feed additives for its broad antibacterial spectrum, well curative effects and low cost. But it easily causes toxicant side effect during application process, especially in large and non-regulate using, causing Gentamicin residues exceeded in animal derived food, constituting a risk to human health, cranial nerve, hearing and kidney damage. Current methods of detection Gentamicin, such as HPLC or GC-MS, are with sample preparation complex, expensive, difficult and time-consuming. While using the Gentamicin ELISA kit is both rapidly and accurately detect Gentamicin residues in samples. This kit is a detection product developed based on ELISA technology, with operation time as short as 50 min and a sensitivity of 0.3 ppb, and linear range from 0.3 ppb to 24.3 ppb