Description
Description: This assay employs a two-site sandwich ELISA to quantitate CENPI in samples. An antibody specific for CENPI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CENPI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CENPI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CENPI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Centromere protein I is involved in the response of gonadal tissues to follicle-stimulating hormone. This gene is also a potential candidate for human X-linked disorders of gonadal development and gametogenesis.By database searching with a sequence identified near the DRP2 gene on chromosome Xq22, Roberts et al. (1996) found that the sequence showed similarity to the rat LRPR1 gene. The deduced 756-amino acid human protein shares 72% sequence identity with the rat protein.Roberts et al. (1996) identified the LRPR1 gene on chromosome Xq22. They found by hybridization to YACs and to cosmids isolated from that region that LRPR1 is transcribed in a proximal-to-distal direction and lies approximately 40 kb proximal to DRP2, while BTK, the gene that is mutant in X-linked agammaglobulinemia, lies approximately 300 kb distal