Description
Description: This assay employs a two-site sandwich ELISA to quantitate HEMGN in samples. An antibody specific for HEMGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HEMGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HEMGN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HEMGN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The deduced mouse and human proteins contain 503 and 484 amino acids, respectively, and share 43% identity. A coiled-coil region and bipartite nuclear localization signal are conserved in the N termini of both proteins. Northern blot analysis detected a major 2.4-kb transcript and a minor 1.8-kb transcript in adult human bone marrow and fetal liver. HEMGN high expression in untreated and mitogen-treated K562 cells, adult bone marrow, and CD34-positive progenitor cells. Lower expression was detected in a child thymus and in a histiocyte lymphoma cell line, but no expression was detected in cultured T cells, monocytes, and nonhematopoietic cell lines examined. Hemgn was expressed in developing hepatic primordia at day 10.5 and, from day 11.5, it was expressed exclusively in fetal liver