Description
Description: This assay employs a two-site sandwich ELISA to quantitate YPEL2 in samples. An antibody specific for YPEL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YPEL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YPEL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YPEL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: YPEL2 gene contains 5 exons and spans about 70 kb. The deduced 119-amino acid protein shares 100% amino acid identity with mouse and monkey Ypel2 and 45% identity with Drosophila Yippee. YPEL2 shares 85.0 to 96.6% amino acid identity with YPEL1, YPEL3, and YPEL4. All YPEL proteins contain an 86-amino acid YPEL consensus sequence. PCR analysis detected YPEL2 expression in adult heart, kidney, lung, pancreas, placenta, skeletal muscle, leukocytes, prostate, spleen, and testis, and in fetal brain, heart, kidney, liver, lung, skeletal muscle, and spleen. Immunofluorescent staining of COS-7 green monkey kidney cells localized monkey Ypel2 to the centrosome and nucleolus during interphase and at several punctate structures around the mitotic apparatus during the mitotic phase