Description
Description: This assay employs a two-site sandwich ELISA to quantitate LMO1 in samples. An antibody specific for LMO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LMO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LMO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LMO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Rhombotin-1 is a protein encoded by the LMO1 gene.LMO1 enocdes a cysteine-rich, two LIM domain transcriptional regulator. It is mapped to an area of consistent chromosomal translocation in chromosome 11, disrupting it in T-cell leukemia, although more rarely than the related gene, LMO2 is disrupted.Boehm et al. (1988) and Greenberg et al. (1989) studied a previously identified translocation associated with T cells in acute lymphoblastic leukemia. The translocation involves the TCR-delta gene on 14q11 and also a transcribed sequence on 11p15. They found that the gene on 11p15 is normally expressed in rhombomeres of the developing hindbrain. The gene product, designated rhombosine or rhombotin, has 155 amino acids and no homology to any previously described protein