Description
Intended Uses: For the quantitative detection of Mouse Prestin (SLC26A5) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SLC26A5 in Mouse serum, plasma. An antibody specific for SLC26A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC26A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC26A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC26A5 bound in the initial step. The color development is stopped and the intensity of the color is measured