Description
Description: This assay employs a two-site sandwich ELISA to quantitate DERL1 in samples. An antibody specific for DERL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DERL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DERL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DERL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Derlin-1 is a widely expressed protein, with strong signals obtained by immunoblotting from liver, spleen, pancreas, lung, thymus, and ovary. Despite the presence of Derlin-1 sequences in brain cDNA libraries, immunoreactivity was not detected in brain.Derlin-1 as an ER membrane protein essential for US11-mediated dislocation of the major histocompatibility complex (MHC) class I heavy chain from the ER to the cytosol, and showed that Derlin-1 mediates the degradation of 2 different type 1 membrane proteins, class I MHC heavy chain and US2.The central component of the complex, Derlin-1, was found to associate with different substrates as they move through the membrane. Inactivation of Derlin-1 in C. elegans caused ER stress