Description
Description: This assay employs a two-site sandwich ELISA to quantitate GLE1 in samples. An antibody specific for GLE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: GLE1Lencodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export.
Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene