Description
Description: This assay employs a two-site sandwich ELISA to quantitate CAPN12 in samples. An antibody specific for CAPN12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAPN12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAPN12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAPN12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Calpains are a family of cytosolic calcium-activated cysteine proteases involved in a variety of cellular processes including apoptosis, cell division, modulation of integrin-cytoskeletal interactions, and synaptic plasticity. CAPN12 belongs to the calpain large subunit family.Full-length Capn12 encodes a deduced 720-amino acid protein containing 4 domains typical of calpains and the 3 conserved residues essential for catalysis. It also contains all 5 Ca(2+)-binding sequences reported for CAPN2. RT-PCR analysis and in situ hybridization of mouse embryos and various adult tissues detected Capn12 expression only in skin, with expression localized to the cortex of the hair follicle. RT-PCR analysis of mouse back skin showed that Capn12 RNA levels increased during the first half of the hair cycle but were absent from later stages