truChIP Chromatin Shearing Tissue Kit with Formaldehyde 520237 from Covaris LLC

Product Specs
ItemtruChIP Chromatin Shearing Tissue Kit with Formaldehyde
CompanyCovaris LLC
Price Supplier Page View Company Product Page
Catalog Number520237
Quantity1
ApplicationsChIP
Typereagents
TemperatureAmbient
ComponentsKit includes: Formaldehyde 16% Methanol-free Formaldehyde (5x1 mL ampules) Buffer A 10 mL 10X Fixing Buffer Buffer B 10 mL 5X Lysis Buffer Buffer C 5 mL 10X Wash Buffer Buffer D2 10 mL 10X SDS Shearing Buffer Buffer E 6 mL 1X Quenching Buffer Buffer F 0.8 mL 100X Protease Inhibitor Cocktail tissueTUBE (6) TT05M XT tissueTUBE milliTUBE–2 mL (6) milliTUBE–2 mL and cap Product Note: Tubes and the supporting holders/racks are required to be purchased separately depending on Covaris instrument being used

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Covaris LLC

14 Gill St, Unit H

Woburn, Massachusetts 01801-1721

United States

Phone: +1 781-932-3959
Fax: +1 781-932-8705

Email: info@covaris.com

Company Profile

Website: www.covaris.com

(1) Nerve injury inhibits Oprd1 and Cnr1 transcription through REST in primary sensory neuronsScientific ReportsNovember 4, 2024Ashok Subedi, Asieh Etemad, Aadhya Tiwari, Yuying Huang, Biji Chatterjee, Samantha M. McLeod, Yungang Lu, DiAngelo Gonzalez, Krishna Ghosh, Mario Sirito, et al.tissues from 5 animals (10 DRGs) per group were collected. qChIP was performed using Covaris truChIP (#520237) and Cell Signaling SimpleChIP (#56383) kits as described by the manufacturer with some modifications.

(2) Nerve injury augments Cacna2d1 transcription via CK2-mediated phosphorylation of the histone deacetylase HDAC2 in dorsal root gangliaThe Journal of biological chemistryNovember 1, 2024Ghosh K, Huang Y, Chen SR, Pan HL.previously described method (11, 60). The truChIP Chromatin Shearing Tissue Kit with formaldehyde (#520237, Covaris) was used to shear chromatin from the DRG tissues of sham and SNL rats. In brief, L5 and L6

(3) Tau protein modulates an epigenetic mechanism of cellular senescence in human SH-SY5Y neuroblastoma cellsFrontiers in Cell and Developmental BiologyOctober 3, 2023Claudia Magrin, Martina Bellafante, Martina Sola, Ester Piovesana, Marco Bolis, Luciano Cascione, Sara Napoli, Andrea Rinaldi, Stéphanie Papin, Paolo Paganettifollowed by 5 min with 125 mM glycine. Cells were washed twice with cold PBS and lysed (Buffer B; PN 520237, Covaris). Nuclei were washed (Buffer C; PN 520237, Covaris) and resuspend in sonication buffer (10

(4) Single-cell multiomics reveals the complexity of TGFβ signalling to chromatin in iPSC-derived kidney organoidsCommunications biologyNovember 27, 2022Davis JL, Kennedy C, Clerkin S, Treacy NJ, Dodd T, Moss C, Murphy A, Brazil DP, Cagney G, Brougham DF, et al.from 5 × 107 cells was prepared for shearing using the Covaris truChIP Shearing Kit (Covaris, cat. no. 520237) according to manufacturer’s protocol. Chromatin was sheared in a pre-cooled AFA microtube using a Covaris

(5) Suppression of p53 response by targeting p53-Mediator binding with a stapled peptideCell reportsApril 5, 2022Allen BL, Quach K, Jones T, Levandowski CB, Ebmeier CC, Rubin JD, Read T, Dowell RD, Schepartz A, Taatjes DJ.otherwise noted. Shearing was performed based on the Covaris truChIP Chromatin Shearing Kit (Covaris: PN 520237) and sheared in 1 mL of Covaris D3 buffer for 8 min, with a duty factor 5% and peak power of 75,200

(6) The Δ40p53 isoform inhibits p53-dependent eRNA transcription and enables regulation by signal-specific transcription factors during p53 activationPLoS biologyAugust 1, 2021Levandowski CB, Jones T, Gruca M, Ramamoorthy S, Dowell RD, Taatjes DJ.for shearing based on the Covaris truChIP Chromatin Shearing Kit (Covaris: (Woburn, Massachusetts) PN 520237) and sheared for 11 minutes with Covaris M220 Focused-ultrasonicator. A total of 25 μL of Protein G

(7) Adverse Maternal Environment Alters MicroRNA-10b-5p Expression and Its Epigenetic Profile Concurrently with Impaired Hippocampal Neurogenesis in Male Mouse HippocampusDevelopmental neuroscienceJanuary 1, 2021Ke X, Huang Y, Fu Q, Lane RH, Majnik A.Chromatin Immunoprecipitation Assay Hippocampal chromatin was isolated from P21 old mice using truChIPTM chromatin shearing tissue kit (#520237, Covaris Inc, MA, USA) and focused-ultrasonicator M220 (Covaris Inc.). Chromatin immunoprecipitation

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truChIP Chromatin Shearing Tissue Kit with Formaldehyde from Covaris LLC

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Covaris LLC

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truChIP Chromatin Shearing Tissue Kit with Formaldehyde from Covaris LLC

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