truChIP Chromatin Shearing Kit with Formaldehyde 520154 from Covaris LLC

Product Specs
ItemtruChIP Chromatin Shearing Kit with Formaldehyde
CompanyCovaris LLC
Price Supplier Page View Company Product Page
Catalog Number520154
Quantity1
ApplicationsChIP
Typereagents
TemperatureAmbient
ComponentsKit includes: Formaldehyde 16% Methanol-free Formaldehyde (5x1 mL ampules) Buffer A 7.5 mL 10X Fixing Buffer Buffer B 5 mL 5X Lysis Buffer Buffer C 2.5 mL 10X Wash Buffer Buffer D3 6 mL 10X SDS Shearing Buffer Buffer E 6 mL 1X Quenching Buffer Buffer F 0.8 mL 100X Protease Inhibitor Cocktail Product Note: Tubes and the supporting holders/racks are required to be purchased separately depending on Covaris instrument being used

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Covaris LLC

14 Gill St, Unit H

Woburn, Massachusetts 01801-1721

United States

Phone: +1 781-932-3959
Fax: +1 781-932-8705

Email: info@covaris.com

Company Profile

Website: www.covaris.com

(1) BACH2 dosage establishes the hierarchy of stemness and fine-tunes antitumor immunity in CAR T cellsNature ImmunologyJanuary 16, 2026Taidou Hu, Ziang Zhu, Ying Luo, Safuwra Wizzard, Jonathan Hoar, Sejal S. Shinde, Kiddist Yihunie, Chen Yao, Tuoqi WupMIG-BACH2 (human) were used to generate ChIP–seq libraries. A truChIP Chromatin Shearing kit (Covaris, 520154) was used for chromatin cross-linking, nuclei isolation and chromatin shearing. Ten micrograms of anti-BACH2

(2) Microbiota-derived inosine programs protective CD8+ T cell responses against influenza in newbornsCellAugust 7, 2025Joseph Stevens, Erica Culberson, Jeremy Kinder, Alicia Ramiriqui, Jerilyn Gray, Madeline Bonfield, Tzu-Yu Shao, Faris Al Gharaibeh, Laura Peterson, Shelby Steinmeyer, et al.transport inhibitor cocktail,E Bioscience,4980-93 truChIP Chromatin Shearing Kit with Formaldehyde,Covaris,520154 v3 Single Cell Reagent Kit,10x Genomics,PN-1000268 Illumina Nextera DNA Flex library kit,Illumina,20015429 Dual

(3) COP1 Deficiency in BRAFV600E Melanomas Confers Resistance to Inhibitors of the MAPK PathwayCellsJune 25, 2025Ada Ndoja, Christopher M. Rose, Eva Lin, Rohit Reja, Jelena Petrovic, Sarah Kummerfeld, Andrew Blair, Helen Rizos, Zora Modrusan, Scott Martin, et al.filling ligation reactions and sonicated in 1 × Shearing Buffer D3 (truChIP chromatin shearing kit, 520154, Covaris, Woburn, MA, USA) on Covaris LE220 to obtain DNA fragments of ~600 bp. Sheared chromatin was

(4) Deciphering the role of RNA in regulating CTCF's DNA binding affinity in leukemia cellsGenome BiolMay 12, 2025Judith Hyle, Wenjie Qi, Mohamed Nadhir Djekidel, Wojciech Rosikiewicz, Beisi Xu, Chunliang Litemperature, and chromatin was prepared using the Covaris TruChIP Chromatin Shearing Kit (Covaris, 520154). Chromatin was sheared by the Covaris M220 ultrasonicator (duty factor of 10, cycles/burst 200 for

(5) ATF2 phosphorylation is a core transcriptional driver of neuron apoptosisbioRxivMay 10, 2025Jorge Gómez-Deza, Matthew Nebiyou, Lara H. El Touny, Mor R. Alkaslasi, Zhenyu Zuo, Josette J. Wlaschin, Francisco M. Nadal-Nicolas, Anastasia L. Slavutsky, Eliza Y. H. Lloyd, Peter M. Hayashi, et al.ice for 10 minutes. Cells were lysed and sonicated using the truChIP Chromatin Shearing Kit (Covaris 520154) according to the manufacturer’s instructions. Chromatin was sonicated in Covaris milliTUBE 1 ml AFA

(6) Oncogenic activation of SMYD3-SHCBP1 promotes breast cancer development and is coupled with resistance to immune therapyCell Death DisMarch 29, 2025Lihua Mo, Min Deng, Ragini Adhav, Yuni Chan, Josh Haipeng Lei, Sek Man Su, Xin Zhang, Tingting An, Jianlin Liu, Jianjie Li, et al.chromatin was fragmented by sonication using a truChIP Ultra-Low Chromatin Shearing Kit (Covaris, USA, 520154). Antibodies against mouse Flag (target Brca1), ERα and the histone markers H3K4me3 and H3K27Ac were

(7) Evolutionary divergence in CTCF-mediated chromatin topology drives transcriptional innovation in humansNat CommunMarch 26, 2025Xia Wu, Dan Xiong, Rong Liu, Xingqiang Lai, Yuhan Tian, Ziying Xie, Li Chen, Lanqi Hu, Jingjing Duan, Xinyu Gao, et al.performed using the Covaris truChIP-seq chromatin shearing kit (Covaris, Woburn, MA, USA, Cat. No. 520154). Briefly, EB(JC) cells were crosslinked with 1% methanol-free formaldehyde. The nuclei were isolated

(8) RLS-associated MEIS transcription factors control distinct processes in human neural stem cellsScientific ReportsNovember 22, 2024Volker Kittke, Chen Zhao, Daniel D. Lam, Philip Harrer, Wojciech Krezel, Barbara Schormair, Konrad Oexle, Juliane Winkelmannwith DPBS. Samples were henceforth processed with the truChIP™ Chromatin Shearing Kit (Covaris, PN 520154) according to the manufacturer’s protocol (010179 Rev K, July 2017). Isolated nuclei were sonicated

(9) DFF-ChIP: a method to detect and quantify complex interactions between RNA polymerase II, transcription factors, and chromatinNucleic Acids ResearchOctober 14, 2024Benjamin M Spector, Juan F Santana, Miles A Pufall, David H Pricewashed in PBS (Hyclone, SH30028.LS) and then sheared using truChIP Chromatin Shearing Kit (Covaris, 520154) and a Covaris E220 focused ultrasonicator. Shearing time was 8 min for Exp 1 and 10 min for Exp 2 and

(10) Transcriptome analysis reveals EBF1 ablation-induced injuries in cardiac systemTheranosticsAugust 12, 2024Yueheng Wu, Shaoxian Chen, Guiping Huang, Lu Zhang, Liying Zhong, Yi Feng, Pengju Wen, Juli Liurespectively. ChIP was performed according to manuals of truChIP™ Chromatin Shearing Kit (Covaris, PN 520154, USA) and EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit (Millipore, 17-10086, USA). Briefly,

(11) Epigenetic Regulation of DLK1-DIO3 Region in Thyroid CarcinomaCellsJune 8, 2024Letícia F. Alves, Isabelle N. da Silva, Diego C. de Mello, Cesar S. Fuziwara, Sonia Guil, Manel Esteller, Murilo V. Geraldoshearing were performed following the high cell protocol from the TruChIP Chromatin Shearing Kit (PN 520154—Covaris, Woburn, MA, USA). Sixty micrograms of sheared chromatin were diluted with the IP buffer and

(12) Viral reprogramming of host transcription initiationNucleic acids researchMay 22, 2024Ungerleider NA, Roberts C, O'Grady TM, Nguyen TT, Baddoo M, Wang J, Ishaq E, Concha M, Lam M, Bass J, et al.100 million latent cells were crosslinked using a truChIP Chromatin Shearing Kit (Covaris, cat no. 520154) following the manufacturer's protocol, with formaldehyde crosslinking for 10 min. Chromatin was sheared

(13) Morc1 reestablishes H3K9me3 heterochromatin on piRNA-targeted transposons in gonocytesProceedings of the National Academy of SciencesMarch 26, 2024Yuta Uneme, Ryu Maeda, Gen Nakayama, Haruka Narita, Naoki Takeda, Ryuji Hiramatsu, Hidenori Nishihara, Ryuichiro Nakato, Yoshiakira Kanai, Kimi Araki, et al.After incubation on ice for 20 min, pelleted nuclei were resuspended in 1× shearing buffer (Covaris, 520154) and fragmented to ~500 bp with a sonicator (BRANSON, SFX150). Fragmented products diluted with RIPA

(14) Enhancer-promoter activation by the Kaposi sarcoma-associated herpesvirus episome maintenance protein LANACell ReportsMarch 26, 2024Xiang Ye, Lindsey N. Guerin, Ziche Chen, Suba Rajendren, William Dunker, Yang Zhao, Ruilin Zhang, Emily Hodges, John Karijolich,Cell Signaling Technology (CST),Cat# 86652 truChIP Chromatin Shearing Kit with Formaldehyde,Covaris,Part# 520154 Arima-HiC + Kit,Arima,Part# A510008 KAPA Hyper Prep Kit,Roche,cat#07962363001 DNA Clean and Concentrator-5,Zymo,Cat#

(15) Differential contributions of nuclear lamina association and genome compartmentalization to gene regulationNucleusDecember 31, 2023Priyojit Das, Rebeca San Martin, Rachel Patton McCordfragmented using a Covaris M220 Sonicator, and the Covaris truChIP Chromatin shearing kit (Covaris; 520154), according to the manufacturers’ instructions, using 1 ml AFA tubes (Covaris). Briefly, cell pellets

(16) Neuroprotective protein ADNP-dependent histone remodeling complex promotes T helper 2 immune cell differentiationImmunityJuly 11, 2023Ferreira ACF, Szeto ACH, Clark PA, Crisp A, Kozik P, Jolin HE, McKenzie ANJ.kit,Roche,Cat#KK4824 RNeasy Plus Micro kit,Qiagen,Cat#74034 truChIP Chromatin Shearing kit,Covaris,Cat#520154 Foxp3 Staining kit reagents,eBioscience,Cat#00-5523-00 Qiagen MinElute kit,Qiagen,Cat#28004 Kappa

(17) Mitotic bookmarking by SWI/SNF subunitsNatureJune 1, 2023Zhu Z, Chen X, Guo A, Manzano T, Walsh PJ, Wills KM, Halliburton R, Radko-Juettner S, Carter RD, Partridge JF, et al.chromatin immunoprecipitation using a modified protocol from the truChIP® Chromatin Shearing Kit (Covaris # PN 520154). In brief, 20 million cells were washed once with 1.5 ml of cold PBS, resuspended in 1.5 ml of room

(18) Dynamic chromatin accessibility licenses STAT5- and STAT6-dependent innate-like function of T(H)9 cells to promote allergic inflammationNature immunologyJune 1, 2023Son A, Meylan F, Gomez-Rodriguez J, Kaul Z, Sylvester M, Falduto GH, Vazquez E, Haque T, Kitakule MM, Wang C, et al.(https://david.ncifcrf.gov/). ChIP-seq sample and library preparation ChIP-seq sample preparation was performed with Covaris kit (PN 520154) as previously described16. Naïve T cells were cultured in vitro under Th9 promoting conditions and

(19) Epigenetic effects of high-fat diet on intestinal tumorigenesis in C57BL/6J-Apc Min/+ miceJournal of Translational Genetics and GenomicsFebruary 23, 2023Dan C. Qu, Devin Neu, Zain Q. Khawaja, Ruoyu Wang, Cynthia F. Bartels, Katreya Lovrenert, Ernest R. Chan, Anne E. Hill-Baskin, Peter C. Scacheri, Nathan A. Bergerintestinal epithelia sample as previously described[10]. The Covaris truChIP™ Chromatin Shearing Kit (520154; Covaris; Woburn, MA) was used to cross-link the intestinal epithelia and extract their cell nuclei

(20) Antagonism among DUX family members evolved from an ancestral toxic single homeodomain proteinbioRxivJanuary 25, 2023Darko Bosnakovski, Erik A. Toso, Elizabeth T. Ener, Micah D. Gearhart, Lulu Yin, Felipe F. Lüttmann, Alessandro Magli, Ke Shi, Johnny Kim, Hideki Aihara, et al.chromatin was isolated and sheared to 200–700 bp using the truChIP Chromatin Shearing Kit (Covaris, 520154) and a Covaris E220evolution focused-ultrasonicator following the manufacturer’s recommendations. Subsequently,

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truChIP Chromatin Shearing Kit with Formaldehyde from Covaris LLC

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truChIP Chromatin Shearing Kit with Formaldehyde from Covaris LLC

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