Description
The Eagle Biosciences Human Anti-Müllerian Hormone ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human Anti-Müllerian Hormone in serum or heparin plasma samples. The assay utilizes the two-site “sandwich” technique with two selected antibodies that bind to different epitopes of human AMH.Assay calibrators, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to N-terminal AMH along with another AMH specific antibody labeled with horseradish peroxidase (HRP). After an initial incubation period, the plate is washed a “sandwich” of solid-phase antibody – human AMH–HRP-conjugated monoclonal antibody” is formed. The unbound monoclonal antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human calprotectin in the test sample. A standard curve is generated by plotting the absorbance versus the respective human calprotectin concentration for each standard on a Cubic or point-to-point curve fitting. The concentration of human AMH in test samples is determined directly from this calibration curve