Nano-Glo HiBiT Blotting System N2410 from Promega

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ItemNano-Glo HiBiT Blotting System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN2410
Quantity100ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) FAM134B isoform 2/RETREG1-2 defines a calnexin-TOLLIP-coupled ER-phagy pathway that restricts Ebola virus glycoprotein and is antagonized by VP40 through macro-autophagybioRxivApril 2, 2026Jing Zhang, Tao Wang, Jiaxin Wen, Jing Lan, Sunan Li, Yong-Hui Zhengmembrane. After that, proteins with HiBiT-tag were detected by Nano-Glo® HiBiT Blotting System (Promega, N2410). Briefly, the membrane was incubated with LgBiT/buffer solution (Promega, N2421) for 2 h at room temperature

(2) Development of a HiBiT-tagged reporter Akabane virus and its application in antiviral drug screeningFrontiers in Cellular and Infection MicrobiologyJanuary 14, 2026Tianai Zhang, Zhongyin Liu, Yao Zhao, Jiamin Qin, Shucheng Zong, Wenke Ruan, Di Wang, Dongjie Chen, Shengkui XuChina). The Nano-Glo® HiBiT Lytic Detection System and Nano-Glo® HiBiT Blotting System kits (N3030、N2410) were purchased from Promega. The ClonExpress® II One Step Cloning Kit (C112-01) were purchased from

(3) Characterization of humoral and cellular immunity induced by mRNA vaccines expressing norovirus VP1 proteins in mice and NHPsMolecular TherapyJanuary 7, 2026Jiajie Wei, Karin Bystol, Leah Alabanza, Shuhui Lim, Jamie Greig, Pooja Gopal, Judith M. Reyes Ballista, Jennifer D. Galli, Christopher Warren, Andrew Swartz, et al.membrane, which was subsequently probed using the Nano-Glo HiBiT blotting kit (Promega, catalog no. N2410) to detect the VP1 constructs and anti-HSP90 (Cell Signaling Technology, catalog no. 5174) to control

(4) The Endo-GeneScreen platform identifies drug-like probes that regulate endogenous protein levels within physiological contextsNat CommunDecember 9, 2025Preston Samowitz, Laszlo Radnai, Thomas Vaissiere, Sheldon D. Michaelson, Camilo Rojas, Ryan Mitchell, Murat Kilinc, Austin Edwards, Justin Shumate, Richard Hawkins, et al.Direct nBIT luciferase signal was also measured using the Nano-Glo® HiBiT Blotting System (Promega: N2410). Following chemiluminescence detection, 30% H2O2 was added to the membrane to quench the chemiluminescence

(5) Profiling of terminating ribosomes reveals translational control at stop codonsbioRxivSeptember 22, 2025Longfei Jia, Yuanhui Mao, Saori Uematsu, Xinyi Ashley Liu, Leiming Dong, Leonardo Henrique França França de Lima, Shu-Bing QianR1018 Nano-Glo HiBiT Lytic Detection System,Promega,Cat# N3040 Nano-Glo HiBiT Blotting System,Promega,Cat# N2410 SuperScript III Reverse Transcriptase,Thermo Fisher Scientific,Cat#18080-044 Deposited data,Deposited

(6) Glucocorticoid receptor and SUMO fluctuations in response to pulsatile glucocorticoids in vitro and in male rat brainsEndocrinologySeptember 17, 2025Caroline A Rivers, Heyam A Abdulqayoom, Yvonne M Kershaw, Oliver R Troy, Zidong Zhao, Becky L Conway-Campbell, Stafford L Lightmanproteins. Immunoblots were probed for the 11–amino acid sequence tag HiBiT using Nano-Glo® HiBiT Blotting (N2410 Promega, Madison, USA) and imaged with an Azure 600 Imager (Azure Biosystems, Dublin, USA). Rat brain

(7) Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activityCell Chemical BiologyJuly 17, 2025Flore Nardella, Tiantian Jiang, Lushun Wang, Monica J. Bohmer, Subhoja Chakraborty, John Okombo, Jaeson Calla, Tatiane Macedo Silva, Samuel Pazicky, Jianwei Che, et al.Bracchi-Ricard et al. 31,N/A Critical commercial assays,, Nano-Glo Hibit Blotting system,Promega,Cat# N2410 Nano-Glo Hibit Lytic system,Promega,Cat# N3030 Renilla-Glo(R) Luciferase Assay System,Promega,Cat#

(8) Monitoring mutant myocilin secretion and localization in trabecular meshwork cell cultures using a protein complementation-based luminescence assayExperimental Eye ResearchJune 1, 2025Hannah A. Youngblood, Ethan F. Harris, Kaylee P. Lankford, Victoria Garfinkel, John D. Hulleman, Raquel L. Liebermansoluble and aggregated fractions could be detected with the HiBiT blotting system (Promega, Cat no. N2410) and yield results comparable to those seen with the immunoblot technique. After spent media was collected,

(9) A general assay platform to study protein pharmacology using ligand-dependent structural dynamicsNat CommunMay 10, 2025Daniel A. Ciulla, Patricia K. Dranchak, Mahesh Aitha, Renier H. P. van Neer, Divia Shah, Ravi Tharakan, Kelli M. Wilson, Yuhong Wang, John C. Braisted, James Ingleseanti-DHFR antibody (Abcam, Cat # ab124814; Clone# EPR5285; RRID:AB_10975115) and NanoBlot (Promega, Cat # N2410) analysis as described in Supplementary Fig. 13. Thermal shift profiles (Thermofluor assay) Thermal

(10) Regulation of human interferon signaling by transposon exonizationCellDecember 26, 2024Giulia Irene Maria Pasquesi, Holly Allen, Atma Ivancevic, Arturo Barbachano-Guerrero, Olivia Joyner, Kejun Guo, David M. Simpson, Keala Gapin, Isabella Horton, Lily L. Nguyen, et al.in-frame insertions by PCR, Sanger sequencing and NanoGlo blot (Nano-Glo® HiBiT Blotting System; Promega #N2410) against the HiBiT tag. CRISPR deletions We generated CRISPR-mediated knockout of IFNAR2-S, IFNAR2-L,

(11) NanoLuciferase technology-based detection of TMPRSS2 as attempt to develop anti-coronavirus agentsBiochemistry and Biophysics ReportsSeptember 1, 2024Yanwen Chen, Yunqi Li, Ye Zhao, Lei Pei, Ling Zhang, Duowu ZouCell Viability Assay (G9243), Nano-Glo HiBiT Lytic Detection System (N3040), Nano-Glo HiBiT Blotting System (N2410), and HiBiT CMV-neo Flexi Vectors (N2401, N2391), were supplied by Promega and employed in this study

(12) GSK3 inhibition reduces ECM production and prevents age-related macular degeneration–like pathologyJCI InsightAugust 8, 2024Sophia M. DiCesare, Antonio J. Ortega, Gracen E. Collier, Steffi Daniel, Krista N. Thompson, Melissa K. McCoy, Bruce A. Posner, John D. Hullemanincubated with blotting buffer containing 1:200 LgBiT for 1–2 hours, with rocking at room temperature (N2410, Promega). NanoGlo substrate was added (1:500), and the blots were incubated for 5–10 minutes (room

(13) A cellular reporter system to evaluate endogenous fetal hemoglobin induction and screen for therapeutic compoundsHemaSphereAugust 6, 2024Thijs C. J. Verheul, Nynke Gillemans, Kerstin Putzker, Rezin Majied, Tingyue Li, Memnia Vasiliou, Bert Eussen, Annelies de Klein, Wilfred F. J. van IJcken, Emile van den Akker, et al.GloMax plate reader (Promega). Western blots were probed using the Nano‐Glo HiBiT blotting assay (#N2410; Promega). SNP arrays HUDEP2 DNA was used for the Global Screening Array (GSAv3); data were analyzed

(14) Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κBCellApril 25, 2024Lampson BL, Ramίrez AS, Baro M, He L, Hegde M, Koduri V, Pfaff JL, Hanna RE, Kowal J, Shirole NH, et al.HiBIT blots, proteins were visualized using the Nano-Glo HiBIT Blotting System (Promega, Catalog No. N2410). Briefly, immediately after transfer, unblocked nitrocellulose membranes were incubated in Nano-Glo

(15) E3 ubiquitin ligase ZBTB25 suppresses beta coronavirus infection through ubiquitination of the main viral protease MProThe Journal of biological chemistryDecember 1, 2023Lear TB, Boudreau ÁN, Lockwood KC, Chu E, Camarco DP, Cao Q, Nguyen M, Evankovich JW, Finkel T, Liu Y, et al.Lytic (catalog no.: N3030), CellTiter-Glo (catalog no.: G9241), Nano-Glo HiBiT Blotting (catalog no.: N2410), pFC37K-HiBiT plasmid (catalog no.: N2391) were from Promega. Anti-ZBTB25 (catalog no: 25631-1-AP)

(16) Single-molecule imaging reveals distinct elongation and frameshifting dynamics between frames of expanded RNA repeats in C9ORF72-ALS/FTDNature communicationsSeptember 11, 2023Latallo MJ, Wang S, Dong D, Nelson B, Livingston NM, Wu R, Zhao N, Stasevich TJ, Bassik MC, Sun S, et al.(1:1000, proteintech, #10582-1-AP).For HiBiT tag detecting, Nano-Glo® HiBiT Blotting System (Promega, N2410) was used according to the manufacturer’s instructions. Uncropped blots for Fig. 5b and Supplementary

(17) Isoform-specific, Semi-quantitative Determination of Highly Homologous Protein Levels via CRISPR-Cas9-mediated HiBiT TaggingBio ProtocJuly 20, 2023Kristina Seiler, Sreoshee Rafiq, Mario P. Tschancatalog number: 11697498001), store at -20 °CNano-Glo® HiBiT blotting system (Promega, catalog number: N2410), store at -20 °CWhite microplates suitable for luminescence4%–20% Mini-PROTEAN® TGC Stain-FreeTM protein

(18) Isoform-specific, Semi-quantitative Determination of Highly Homologous Protein Levels via CRISPR-Cas9-mediated HiBiT TaggingBIO-PROTOCOLJuly 20, 2023Kristina Seiler, Sreoshee Rafiq, Mario P. Tschancatalog number: 11697498001), store at -20 °CNano-Glo® HiBiT blotting system (Promega, catalog number: N2410), store at -20 °CWhite microplates suitable for luminescence4%–20% Mini-PROTEAN® TGC Stain-FreeTM protein

(19) Use of the dTAG system in vivo to degrade CDK2 and CDK5 in adult mice and explore potential safety liabilitiesToxicological SciencesJune 28, 2023Paul Yenerall, Tae Sung, Kiran Palyada, Jessie Qian, Seda Arat, Steven W Kumpf, Shih-Wen Wang, Kathleen Biddle, Carlos Esparza, Stephanie Chang, et al.above. After probing with all antibodies, the Nano-Glo HiBit Blotting System (Promega, catalog no. N2410) was used to visualize HiBit expression. In brief, following the manufacturer’s recommendations on volumes

(20) A luminescence-based reporter to study tau secretion reveals overlapping mechanisms for the release of healthy and pathological tauFrontiers in NeuroscienceJune 5, 2023Dianne Marquez Lopez, Connor J. Maltby, Hannah Warming, Nullin Divecha, Mariana Vargas-Caballero, Mark J. Coldwell, Katrin Deinhardtanalyze the image. To detect HiBiT, membranes were incubated in Nano-Glo HiBiT Blotting Buffer (Promega, N2410) containing LgBiT followed by addition of furimazine, and imaged using a luminescence imager with a

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Nano-Glo HiBiT Blotting System

Nano-Glo HiBiT Blotting System from Promega

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Nano-Glo HiBiT Blotting System from Promega

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