Nano-Glo HiBiT Extracellular Detection System N2421 from Promega

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ItemNano-Glo HiBiT Extracellular Detection System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN2421
Quantity100ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) FAM134B isoform 2/RETREG1-2 defines a calnexin-TOLLIP-coupled ER-phagy pathway that restricts Ebola virus glycoprotein and is antagonized by VP40 through macro-autophagybioRxivApril 2, 2026Jing Zhang, Tao Wang, Jiaxin Wen, Jing Lan, Sunan Li, Yong-Hui ZhengNano-Glo® HiBiT Blotting System (Promega, N2410). Briefly, the membrane was incubated with LgBiT/buffer solution (Promega, N2421) for 2 h at room temperature with gentle rocking and then at 4 °C overnight. The membrane was incubated

(2) A dual topology of STIM1 at the plasma membrane regulates calcium constitutive entryCell Mol Life SciMarch 6, 2026Paul Buscaglia, Nelig Le Goux, Patrice Hemon, Anthony Mainguy, Maela Hus, Mathieu Gimaray, Paul Roger Claude Imbert, Alix A. J. Rouault, Julien A. Sebag, Olivier Mignenwith 100 μL of HiBiT complementation reagent (Promega, Nano-Glo HiBiT extracellular detection system, N2421) following commercial recommendations and containing a final concentration of 200 nmol/L LrgBiT protein.

(3) Protocols for Monitoring Unconventional Protein Secretion Using Luminescence and Trapping ApproachesCurr ProtocFebruary 16, 2026Eloïse Néel, Morgane Denus, William Fargues, Charline Gal, Camille Enjolras, Ana Boulanger, Marie-Laure Parmentier, Julien Villeneuvelytic substrate, and LgBiT proteinNano‐Glo HiBiT Extracellular Detection System (Promega, cat. no. N2421), including extracellular buffer, extracellular substrate, and LgBiT proteinLDH activity assay kit (Thermo Fisher Scientific

(4) In silico docking yields small molecule negative allosteric modulators targeting the core of Frizzled 7Nat CommunDecember 14, 2025Magdalena M. Scharf, Julia Kinsolving, Lukas Grätz, Jan Hendrik Voss, David Carrasco-Busturia, Björn Forsberg, Peter Kolb, Gunnar SchulteN1110) or a mix of LgBiT and furimazine (for HiBiT-FZD7, both diluted 1/200 in HBSS, Promega, cat.-No.: N2421) was added, the plate was incubated for 15 min in the dark and BRET was measured five times using a

(5) Reduced Fc-mediated antibody responses after COVID-19 mRNA vaccination in a cohort of people living with HIV-1Scientific ReportsNovember 25, 2025Jéromine Klingler, Priyanka Gadam Rao, Juan C. Bandres, Ismael Pena, Katherine Bolanos Roldan, Gagandeep Singh, Brian Monahan, Charles Gleason, Yuexing Chen, Stefan Slamanig, et al.After 4 h, luminescence was measured using Nano-Glo® HiBiT Extracellular assay detection (Promega, #N2421) and ADCC scores were calculated relative to digitonin (Sigma, #D141), used as maximum release control. Ethics

(6) Autophagosome marker, LC 3, is released extracellularly via several distinct pathwaysFEBS Open BioNovember 10, 2025Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita,Thermo Fisher Scientific,H1399,1:1000,IF Reagent,Reagent,Reagent Nano Glo HiBiT Extracellular Detection System,Promega,Cat#N2421 Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines HEK293T,ATCC,Cat#

(7) Real-time monitoring of secretory protein traffic for investigating trafficking regulators of ACE2 in living cellsJournal of Biological ChemistrySeptember 1, 2025Chang Zhang, Mengxin Yang, Yanzun An, Muyi Liu, Huilong Li, Ruzhou Zhao, Xiaopan Yang, Linfei Huang, Yanhong Zhang, Renjun Peng, et al.incubation for adhesion. For HiBiT signal detection, Nano-Glo HiBiT Extracellular Detection Reagent (N2421; Promega) was prepared by mixing Large BiT (1:200 dilution) and furimazine substrate (1:100 dilution)

(8) Multivalent interactions with CCR4-NOT and PABPC1 determine mRNA repression efficiency by tristetraprolinNat CommunAugust 13, 2025Filip Pekovic, Wi S. Lai, Joshua Corbo, Stephanie N. Hicks, Keiko Luke, Perry J. Blackshear, Eugene Valkovwells of black 96-well plates (Thermo Scientific 137101). An aliquot of 75 µl Nano-Glo mixture (Promega N2421), consisting of 75 µl of Nano-Glo HiBit Extracellular Buffer and the LgBit Protein (1:100) and the Nano-Glo

(9) Biased agonism of GLP-1R and GIPR enhances glucose lowering and weight loss, with dual GLP-1R/GIPR biased agonism yielding greater efficacyCell Rep MedJune 17, 2025Ruben Rodriguez, Anne Hergarden, Shyam Krishnan, Marikris Morales, Davina Lam, Ted Tracy, Teresa Tang, Avalon Patton, Craig Lee, Asmita Pant, et al.DiscoverX,90-0075LM Nano-Glo® Live Cell Substrate,Promega,N2012 Nano-Glo® HiBiT Extracellular Detection System,Promega,N2421 Fugene 6,Promega,E2692 U-PLEX Mouse Insulin Assay,Meso Scale Discovery,K1526HK-2 ,, Experimental models

(10) A Sensitive and Versatile Cell-Based Assay Combines Luminescence and Trapping Approaches to Monitor Unconventional Protein SecretionTrafficMay 21, 2025Morgane Denus, Aurore Filaquier, William Fargues, Eloïse Néel, Sarah E. Stewart, Maëlle Colladant, Thomas Curel, Alexandre Mezghrani, Philippe Marin, Sylvie Claeysen, et al.(Bio‐Rad, #170‐5061 and GeneTex, #GTX14698), Nano‐Glo HiBit Extracellular Detection system (Promega, #N2421), Nano‐Glo HiBit Lytic Detection system (Promega, #N3040), LDH Assay Kit (Abcam, #ab102526), Annexin V‐FITC Apoptosis Staining/Detection Kit

(11) Carbonic anhydrase inhibition ameliorates tau toxicity via enhanced tau secretionNature chemical biologyApril 1, 2025Lopez A, Siddiqi FH, Villeneuve J, Ureshino RP, Jeon HY, Koulousakis P, Keeling S, McEwan WA, Fleming A, Rubinsztein DC.the Nano-Glo HiBit Extracellular Detection System kit, according to the manufacturer’s instructions (N2421, Promega). This protocol is based on the established concept of bimolecular fluorescence complementation

(12) The pneumococcal bacteriocin streptococcin B is produced as part of the early competence cascade and promotes intraspecies competitionmBioFebruary 5, 2025J. D. Richardson, Emily Guo, Ryan M. Wyllie, Paul Jensen, Suzanne Dawid96-well plate (Costar, 3917) following the addition of HiBiT Extracellular Detection Reagent (Promega, N2421) at a 1:1 ratio. For endpoint assays, each sample was quantified with three technical replicates and

(13) Investigating NanoLuc-EGFR engineered cell lines for real-time monitoring of EGFR protein dynamics in live cellsBiochemical and Biophysical Research CommunicationsNovember 1, 2024Ranjit K. Mehta, Mingjia Tan, Mohammed K. Hassan, Tengda Zhao, David M. Markovitz, Theodore S. Lawrence, Mukesh K. Nyatirecommended dilution of 1:100 for Nano-Glo HiBiT extracellular and lytic detection reagents (Promega, N2421 and N3030) was suboptimal for the A549-HiBiT EGFR cell line model. We refined the protocol to a 1:400

(14) Hijacking of internal calcium dynamics by intracellularly residing viral rhodopsinsNature CommunicationsJanuary 2, 2024Ana-Sofia Eria-Oliveira, Mathilde Folacci, Anne Amandine Chassot, Sandrine Fedou, Nadine Thézé, Dmitrii Zabelskii, Alexey Alekseev, Ernst Bamberg, Valentin Gordeliy, Guillaume Sandoz, et al.luminescence measurements of oocytes were performed using the kit Nano-Glo HiBiT Extracellular Detection System (#N2421, Promega) by adding to each well 100 μl of the Nano-Glo HiBiT extracellular reagent. After 10 minutes

(15) UBE3C Facilitates the ER-Associated and Peripheral Degradation of Misfolded CFTRCellsNovember 30, 2023Yuka Kamada, Hazuki Tateishi, Uta Nakayamada, Daichi Hinata, Ayuka Iwasaki, Jingxin Zhu, Ryosuke Fukuda, Tsukasa OkiyonedaExtracellular Nluc Substrate (Promega, Cat# CS313501) or Nano Glo HiBiT Extracellular system (Promega, Cat# N2421), according to the manufacturer’s instructions as previously [23,24,25]. The luminescent signal was

(16) Structural basis of peptide secretion for Quorum sensing by ComANature CommunicationsNovember 7, 2023Lin Yu, Xin Xu, Wan-Zhen Chua, Hao Feng, Zheng Ser, Kai Shao, Jian Shi, Yumei Wang, Zongli Li, Radoslaw M. Sobota, et al.samples were quantified by adding an equal volume of the HiBiT Extracellular Detection Reagent (Promega, N2421) to each well. Luminescence was measured using a Tecan plate reader with the following settings: 1 s

(17) Insulin sensitization by small molecules enhancing GLUT4 translocationCell chemical biologyAugust 17, 2023Yin TC, Van Vranken JG, Srivastava D, Mittal A, Buscaglia P, Moore AE, Verdinez JA, Graham AE, Walsh SA, Acevedo MA, et al.commercial assays,Critical commercial assays Nano-Glo HiBiT extracellular detection system,Promega,Cat# N2421 Nano-Glo HiBiT lytic detection system,Promega,Cat# N3040 Alphalisa insulin kit,Perkin Elmer,Cat# AL3184C Nano-Glo

(18) Engineering Rapalog-Inducible Genetic Switches Based on Split-T7 Polymerase to Regulate Oncolytic Virus-Driven Production of Tumour-Localized IL-12 for Anti-Cancer ImmunotherapyPharmaceuticals (Basel, Switzerland)May 7, 2023Martin NT, Crupi MJF, Taha Z, Poutou J, Whelan JT, Vallati S, Petryk J, Marius R, Austin B, Azad T, et al.used as indicated in the manufacturer’s instructions (NanoGlo HiBiT Extracellular Detection System, N2421; Promega). Human or murine IL-12 functionality was assessed using HEKBlue IL-12 reporter cells (hkb-il12;

(19) Preclinical Evaluation of ON203, A Novel Bioengineered mAb Targeting Oxidized Macrophage Migration Inhibitory Factor as an Anticancer TherapeuticMolecular Cancer TherapeuticsMay 4, 2023Gregor Rossmueller, Irina Mirkina, Barbara Maurer, Verena Hoeld, Julia Mayer, Michael Thiele, Randolf J. Kerschbaumer, Alexander Schinaglprotein. For this, 10 μL/well of the freshly prepared Nano-Glo HiBiT Extracellular Reagent (Promega, # N2421) was added. After incubation for 3 minutes, luminescence was measured on a Tecan Infinite M200 PRO (RRID:SCR_019033)

(20) RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagyNature communicationsOctober 12, 2022Zhang J, Wang B, Gao X, Peng C, Shan C, Johnson SF, Schwartz RC, Zheng YH.Nano-Glo® HiBiT Blotting System (Promega, N2410). Briefly, the membrane was incubated with LgBiT/buffer solution (Promega, N2421) for 2 h at room temperature with gentle rocking and then at 4 °C overnight. The membrane was incubated

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Nano-Glo HiBiT Extracellular Detection System

Nano-Glo HiBiT Extracellular Detection System from Promega

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Nano-Glo HiBiT Extracellular Detection System from Promega

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