Nano-Glo HiBiT Lytic Detection System N3030 from Promega

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ItemNano-Glo HiBiT Lytic Detection System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN3030
Quantity10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

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Website: http://www.promega.com

(1) Hepatitis C Virus 5'UTR Sequences That Bind eIF3 and Ribosomal 40S Subunits Confer Stimulation of Minus-Strand RNA SynthesisInt J Mol SciApril 2, 2026Attiya Qadoos Malik, Lyudmila Shalamova, Mozhdeh Khajouei, Jonas Budnik, Anna-Lena Hell, Elena Jost, Gesche K. Gerresheim, Oliver Rossbach, Michael NiepmannSteady-Glo Luciferase Assay System (Promega, Walldorf, Germany; E2510); Nano-Glo HiBiT Lytic Detection System (Promega; N3030). Non-standard chemicals: Lipofectamine MessengerMax Transfection reagent (Invitrogen; LMRNA008); Acidic

(2) p300-mediated histone H3K18 lactylation promotes mitochondrial ROS accumulation via mitophagy inhibition to potentiate dopamine agonists efficacy in prolactinomasRedox BiologyApril 1, 2026Sihan Li, Qian Jiang, Quanji Wang, Xingbo Li, Zihan Wang, Linpeng Xu, Shuyan Luo, Yaorui Wang, Huaqiu Zhang, Kai Shu, et al.autophagic flux was determined using the Nano-Glo® HiBiT Lytic Detection System (Promega, Cat. No. N3030) with or without bafilomycin A1 (MedChemExpress, Cat. No. HY-100558; final 50 nM, 4 h), and luminescence

(3) A Facile Protocol for C(sp 2 )–C(sp 3 ) Bond Formation Reactions Toward Functionalized E3 Ligase LigandsChemMedChemFebruary 25, 2026Anita Maksutova, Thomas M. Geiger, Lorenzo Cianni, Dominika E. Pieńkowska, Jan Gerhartz, Lina Read, Aleša Bricelj, Alexander Herrmann, Maurice Leon Nelles, Yuen Lam Dora Ng, et al.CO2 for 5 h, and the HiBiT assay was performed as described in the manufacturer's protocol (Promega, N3030). Briefly, 12.5 µL/well premixed HiBiT reagent was added using ClipTip Pipettes (Thermo Fisher) and

(4) Diastereomeric Branched-Ester dBET1 Analogs Exhibit Conformation-Dependent Differences in Passive Membrane PermeabilityJournal of Medicinal ChemistryFebruary 12, 2026Mazin A. S. Abdelwahid, Eisuke Hayakawa, Keigo Hirai, Mayumi Ishii, Kayoko Kanamitsu, Saori Yasuda, Fumiaki Ohtake, Shinichi Sato, Shusuke Tomoshige, Minoru IshikawaMol Cell 2021). HiBiT assay was performed using the Nano-Glo HiBiT Lytic Detection System (Cat. No. N3030, Promega) according to the manufacturer’s protocol. The chemiluminescence intensity was measured using

(5) Expanding the toolbox to develop IAP-based degraders of TEAD transcription factorsCommunications ChemistryJanuary 19, 2026Nishma Gupta, Nicole Trainor, Mona Radwan, Stephanie Nguyen, Luke Duncan, Andrew X. Tang, Julia Beveridge, Natasha Silke, Jumana Yousef, Ceren Bilgilier, et al.before the HiBiT signal was measured by adding 20 µL of Nano-Glo® HiBiT Lytic Detection System (Promega, N3030) prepared according to the manufacturer’s protocol, and incubated at RT for 5–10 min before reading

(6) Development of a HiBiT-tagged reporter Akabane virus and its application in antiviral drug screeningFrontiers in Cellular and Infection MicrobiologyJanuary 14, 2026Tianai Zhang, Zhongyin Liu, Yao Zhao, Jiamin Qin, Shucheng Zong, Wenke Ruan, Di Wang, Dongjie Chen, Shengkui Xu(Beijing, China). The Nano-Glo® HiBiT Lytic Detection System and Nano-Glo® HiBiT Blotting System kits (N3030、N2410) were purchased from Promega. The ClonExpress® II One Step Cloning Kit (C112-01) were purchased

(7) Binding of Glycyl-tRNA synthetase to Mengovirus RNA stimulates translationNucleic Acids ResearchJanuary 8, 2026Fabian Droß, Tim Gödert, Selena A Fuchshuber, Dimitar V Nachev, Dmitri E Andreev, Markus Fricke, Muriel Ritsch, Gesche K Gerresheim, Simeon Repp, Yannic Noe, et al.(Bio-Rad #1 725 271), SNAP-capture magnetic beads (NEB # S9145S), Nano-Glo HiBiT Lytic Reagent (Promega N3030). Biological Resources: Attenuated Mengovirus (MV) genome from plasmid pMC0 [45]; Expression vectors

(8) A subset of type 4 secretion system effectors of Brucella spp. associates to outer membrane vesiclesmicroLifeJanuary 5, 2026Maren Ketterer, Naadir Ganief, Petra Chiquet, Adélie Lannoy, Audrey Verhaeghe, Joanne Braunweiler, Marc Dieu, Xavier De Bolle, Maxime Québatte, Christoph DehioThe pellets were set aside for further analysis using Nano-Glo HiBiT Lytic Detection System (Promega, N3030). 100 µl of the cleared supernatants were dispensed in a white 96-well plate in triplicate and equilibrated

(9) Targeted Inhibition of ELANE Expression Using Adenine Base Editing to Treat Severe Congenital NeutropeniaMolecular Therapy Methods & Clinical DevelopmentDecember 11, 2025Betül Findik, Benjamin Dannenmann, Franka Bernhard, Masako Monika Kaufmann, Sandra Ammann, Sergey Kandabarau, Maksim Klimiankou, Fabian Mauch, Patrick Münzer, Oliver Borst, et al.10% FCS, 1% penicillin/streptomycin) were lysed with 50 μL Nano-Glo HiBiT lytic reagent (Promega, #N3030) for 30 min at room temperature (RT) while shaking. Luminescence signal was quantified using a GloMax

(10) Ubiquitin pathway blockade reveals endogenous ADP-ribosylation marking PARP7 and AHR for degradationEMBO JDecember 1, 2025Andrii Gorelik, Nina Đukić, Rebecca Smith, Chatrin Chatrin, Osamu Suyari, Jason Matthews, Ivan Ahelculture medium. Following drug incubation, Nano-Glo® HiBiT Lytic Detection System was used (Promega, N3030) according to the manufacturer’s instructions. The luminescence was quantified using the SpectraMax M5

(11) A degron-mimicking molecular glue drives CRBN homo-dimerization and degradationNature CommunicationsNovember 19, 2025Gerasimos Langousis, Pablo Gainza, Moritz Hunkeler, Despoina Kapsitidou, Etienne J. Donckele, Stefano Annunziato, Lars Wiedmer, Katherine F. M. Jones, Bradley DeMarco, Chao Quan, et al.Nano-Glo® HiBiT Lytic Reagent was prepared according to the manufacturer’s instructions (Promega Cat# N3030), and 25 μL were dispensed to each well. The Plate was incubated for 10 min at room temperature, and

(12) An AI-driven workflow for the accelerated optimization of cell-free protein synthesisiScienceOctober 17, 2025Mostafa M. Khalil, Aisha Elsawah, An N. Hoang, Jean-Loup Faulon, Baptiste Panthu, Joan Hérissonkit,NEB,Cat#T1130 NucleoBond Xtra Maxi kit,Macherey-Nagel,Cat#740414.1 Nano-Glo® HiBiT Lytic Detection System,Promega,Cat#N3030 Nano-Glo® HiBiT Lytic Detection System,Promega,Cat#N3040 ,, Deposited data,Deposited data,Deposited

(13) Discovery of a Potent and Selective TEAD Degrader with Durable Degradation ActivityAdvanced ScienceOctober 5, 2025Linhui Cao, Jing Yang, Yuhang Liu, Xiaotong Chen, Yufang Shi, Yunshuo Zhao, Yong Zhang, Jian Chen, Bowen Li, Wuqiang Wen, et al.cells were lysed and assayed for degradation using the Nano‐Glo HiBiT Lysis Assay System (Promega, #N3030). Luminescence signals were read using an Envision plate reader (Revvity) and the data were analyzed

(14) Lipid bilayer properties govern substrate engagement and extraction by the AAA+ ATPase Msp1Journal of Biological ChemistryOctober 1, 2025Heidi L. Fresenius, Brian Acquaviva, Deepika Gaur, Baylee A. Smith, Matthew L. Wohleversamples were plated onto white 96-well plates (Corning #07201204) and 20 μl of lytic furimazine (Promega #N3030) was added. Immediately plates were read at 470 nm wavelength to measure luminescence. Extraction assays

(15) Glucocorticoid receptor and SUMO fluctuations in response to pulsatile glucocorticoids in vitro and in male rat brainsEndocrinologySeptember 17, 2025Caroline A Rivers, Heyam A Abdulqayoom, Yvonne M Kershaw, Oliver R Troy, Zidong Zhao, Becky L Conway-Campbell, Stafford L LightmanSanger sequencing (Fig. S1 (38)). HiBiT expression was assayed with Nano-Glo® HiBiT Lytic Detection System (N3030 Promega, Madison, USA) and luminescence measured using a GloMax® microplate reader (Promega, Madison,

(16) Protective antigen–mediated delivery of an anti-CRISPR protein for precision genome editingProceedings of the National Academy of SciencesAugust 12, 2025Axel O. Vera, Nicholas L. Truex, Vedagopuram Sreekanth, Bradley L. Pentelute, Amit Choudhary, Ronald T. Raineslysed in the presence of LgBiT and lytic substrate (Nano-Glo HiBiT Lytic Detection System, Promega, N3030), and their luminescence was quantified (Perkin Elmer EnVison). Cas9-mediated cleavage and HiBiT tag

(17) Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activityCell Chemical BiologyJuly 17, 2025Flore Nardella, Tiantian Jiang, Lushun Wang, Monica J. Bohmer, Subhoja Chakraborty, John Okombo, Jaeson Calla, Tatiane Macedo Silva, Samuel Pazicky, Jianwei Che, et al.assays,, Nano-Glo Hibit Blotting system,Promega,Cat# N2410 Nano-Glo Hibit Lytic system,Promega,Cat# N3030 Renilla-Glo(R) Luciferase Assay System,Promega,Cat# E2750 Deposited data,, Compound 12-selected resistance

(18) Live cell GLUT4 translocation assay reveals Per3 as a novel regulator of circadian insulin sensitivity in skeletal muscle cellsBiology OpenJuly 15, 2025Rashmi Sivasengh, Andrew Scott, Brendan M. GabrielHiBiT detection tool from Promega UK. An equal volume of Nano-Glo HiBiT Reagent (100 µl) (Promega, N3030), consisting of Nano-Glo HiBiT Buffer, Nano-Glo HiBiT Substrate, and LgBiT Protein, was added according

(19) Phosphorylation-activated G protein signaling stabilizes TCP14 and JAZ3 to repress JA signaling and enhance plant immunityMolecular PlantJuly 7, 2025Haiyan Jia, Natalie Hewitt, Lucía Jordá, Tigran M. Abramyan, Josh Tolliver, Janice L. Jones, Kinya Nomura, Jing Yang, Sheng-Yang He, Alexander Tropsha, et al.luciferase signal by cLUC-GPA1-HiBiT protein expression, Nano-Glo® HiBiT Lytic Detection System (Cat#: N3030, Promega) quantified the cLUC-GPA1-HiBiT protein expression level in the N. benthamiana leaf discs.

(20) Chemical and topological design of multicapped mRNA and capped circular RNA to augment translationNature BiotechnologyJuly 1, 2025Hongyu Chen, Dangliang Liu, Abhishek Aditham, Jianting Guo, Jiahao Huang, Franklin Kostas, Kamal Maher, Mirco J. Friedrich, Ramnik J. Xavier, Feng Zhang, et al.Nano-Glo dual-luciferase reporter assay system (Promega, N1610) or Nano-Glo HiBiT lytic detection system (Promega, N3030). mRNA profiling in transfected cell culture or tissue with STARmap and RIBOmap Cell-culture-based

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Nano-Glo HiBiT Lytic Detection System

Nano-Glo HiBiT Lytic Detection System from Promega

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Nano-Glo HiBiT Lytic Detection System from Promega

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