Nano-Glo HiBiT Extracellular Detection System N2420 from Promega

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ItemNano-Glo HiBiT Extracellular Detection System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN2420
Quantity10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Targeted recruitment of USP15 enhances CTLA4 surface levels and restricts its degradationLife Science AllianceApril 1, 2026Francesca Querques, Victoria Ciampani, Pei Yee Tey, Victoria D Kutilek, Elma Kadic, Michael J Clague, Sylvie UrbénM A/C for 18 h. HiBiT assays were performed with the NanoGlo HiBiT extracellular detection system (N2420; Promega) to detect cell surface CTLA4, or the NanoGlo HiBiT Lytic detection system (N3040; Promega)

(2) A pH-driven receptor switch from ACE2 to NPC1 enables endosomal entry of SARS-CoV-2ResearchSquareMarch 4, 2026Yong-Hui Zheng, Zhongshun Liu, Yu Pang, Ilyas Khan, Li Ou, Yixiang Hu, Sunan Li, Justin Richner, Tongqing Zhou4°C. For fusion assays, HiBiT-N VLPs and ACE2-LgBiT EVs were combined with NanoLuc substrate (Promega N2420), 10 μM DrkBiT (NovoPro 319394), and trypsin (1 ng/μL) in 96-well plates. Luminescence was recorded

(3) Omics-Based Expression Cassette for Heterologous Protein Production in Y. lipolyticaACS Synthetic BiologyFebruary 20, 2026Ridwan Elemosho, Chenxi Zhang, Alexandros Karyolaimos, Agata Brambor, Raul Pasadas Castilla, Jonathan Asmund Arnesen, Leonie Johanna Jahn, Morten Otto Alexander Sommerdetection. Protein quantification was done using the HiBiT extracellular detection system (Promega #N2420), and luminescence reads were normalized to the cells’ optical density. SDS-PAGE and HiBiT Blotting SDS-PAGE

(4) The FliI ATPase couples ATP hydrolysis to substrate switching in bacterial flagellar type-III secretionmBioJanuary 14, 2026Rosa Einenkel, Caroline Kühne, Mario Delgadillo‑Guevara, Lasse Hallenga, Christian Goosmann, Marc Erhardtthe split NanoBit enzyme Nano-Glo HiBiT Extracellular Detection System was purchased from Promega (N2420). The quantification of the secreted HiBiT-tagged proteins was determined as described previously (52).

(5) A subset of type 4 secretion system effectors of Brucella spp. associates to outer membrane vesiclesmicroLifeJanuary 5, 2026Maren Ketterer, Naadir Ganief, Petra Chiquet, Adélie Lannoy, Audrey Verhaeghe, Joanne Braunweiler, Marc Dieu, Xavier De Bolle, Maxime Québatte, Christoph Dehioequilibrated to RT. Sterile DM was used as negative control. Nano-Glo HiBiT Extracellular Reagent (Promega, N2420) was prepared following manufacturer’s instructions and 100 µl were dispensed to each sample. The plate

(6) The Endo-GeneScreen platform identifies drug-like probes that regulate endogenous protein levels within physiological contextsNat CommunDecember 9, 2025Preston Samowitz, Laszlo Radnai, Thomas Vaissiere, Sheldon D. Michaelson, Camilo Rojas, Ryan Mitchell, Murat Kilinc, Austin Edwards, Justin Shumate, Richard Hawkins, et al.regulated nBIT activity. Starting with the Nano-Glo® HiBIT Extracellular Detection System (Promega: N2420), a HiBIT control protein (Promega: N3010) was diluted to 100 pM in buffer containing 0.1% BSA-PBS and

(7) Assembly and dynamic regulation of the tip filament of the Bordetella type III secretion system injectisomemBioNovember 12, 2025Ivana Malcova, Martin Zmuda, Jan Valecka, Jana Kamanovaperiod. To assess Bsp22 protein levels, the Nano-Glo HiBiT Extracellular Detection System (Promega, Cat# N2420) was utilized following the manufacturer’s instructions. In brief, suspension (diluted 1:10) was mixed

(8) Functional and intricate interaction network connecting Helicobacter pylori Cag type 4 secretion system surface proteins with outer membrane proteins HopQ and HopZMicrolifeOctober 6, 2025Felix Metz, Johanna Beilmann, Simon H Bats, Andreas Latoscha, Gregor Witte, Remco T A Megens, Karl-Peter Hopfner, Kaisa Thorell, Wolfgang Fischer, Laurent Terradot, et al.CagN-HiBiT detection in Western blot and using Nano-Glo HiBiT Extracellular Detection System (Promega #N2420) for bacterial surface detection of HiBiT-tagged proteins (Fig. S6). Subsequently, plasmids, containing

(9) A CXCR4 partial agonist improves immunotherapy by targeting immunosuppressive neutrophils and cancer-driven granulopoiesisCancer CellAugust 11, 2025Jin Qian, Chenkai Ma, Quin T. Waterbury, Xiaofei Zhi, Christine S. Moon, Ruhong Tu, Hiroki Kobayashi, Feijing Wu, Biyun Zheng, Yi Zeng, et al.562574 MycoAlert Mycoplasma Detection Kit,Lonza,Cat# LT07-118 HiBiT Extracellular Detection System,Promega,Cat# N2420 BD Cytofix/Cytoperm™ kit,"BD Biosciences,",Cat# 554714 BD Phosflow™ Perm Buffer III,BD Biosciences,Cat#

(10) Host protein ARF1 is a proviral factor for SARS-CoV-2 and a candidate broad-spectrum therapeutic targetNat CommunJuly 9, 2025Cunhuan Zhang, Yuan-Qin Min, Heng Xue, Haiyan Zhang, Kunpeng Liu, Yichao Tian, Ziying Yang, Zihan Zhao, Hang Yang, Chao Shan, et al.collected for luminescence signal analysis using Nano-Glo HiBiT Extracellular Detection reagents (Promega, N2420) on Glomax Multi (Promega). ARF1 KD and SARS-CoV-2 infection in K18-hACE2 mice For the production

(11) Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectorsVet ResJune 8, 2025Hana Michova, Jan Pliva, Anezka Jirsova, David Jurnecka, Jana Kamanovaquantification was performed using the Nano-Glo HiBiT Extracellular Detection System (Promega, Cat. No. N2420) as previously described [49]. In brief, for the preparation of cell extracts, cell pellets were resuspended

(12) Conservation and divergence of regulatory architecture in nitrate-responsive plant gene circuitsThe Plant CellJune 4, 2025Chao Bian, Gozde S Demirer, M Tufan Oz, Yao-Min Cai, Sam Witham, G Alex Mason, Zhengao Di, Florian Deligne, Ping Zhang, Rachel Shen, et al.yield was quantified by measuring luminescence with the Nano-Glo HiBiT Extracellular Detection System (N2420, Promega, Madison, WI) and HiBiT Control Protein (N3010, Promega). We were unable to obtain the soluble

(13) Antiviral resistance and barrier integrity at the maternal-fetal interface restrict hepatitis E virus from crossing the placental barrierProceedings of the National Academy of SciencesMay 6, 2025Debin Tian, Wen Li, C. Lynn Heffron, Hassan M. Mahsoub, Bo Wang, Tanya LeRoith, Xiang-Jin Mengby sequencing and evaluated by using a Nano-Glo® HiBiT Extracellular Detection System (Promega, Cat#N2420) according to the manufacturer’s instructions. Anti-HEV Resistance Transfer Assay. The coculture placental

(14) CARTp/GPR160 mediates behavioral hypersensitivities in mice through NOD2PainApril 1, 2025Rachel M. Schafer, Luigino A. Giancotti, John C. Chrivia, Ying Li, Fatma Mufti, Thomas A. Kufer, Jinsong Zhang, Timothy M. Doyle, Daniela Salveminidesigned to detect cells expressing membrane proteins (Nano-Glo HiBiT Extracellular kit Detection system, N2420). The expression of the NL-FLAG-HiBiT GPR160 proteins was validated using Western blots, in conjunction

(15) SARS-CoV-2 Omicron variations reveal mechanisms controlling cell entry dynamics and antibody neutralizationPLOS PathogensDecember 2, 2024Enya Qing, Julisa Salgado, Alexandria Wilcox, Tom Gallagher[10,23,24,59–62]. Briefly, at 4°C, HiBiT-N VLPs and hACE2-LgBiT EVs were mixed with nanoluc substrate (Promega #N2420), DrkBiT (10 μM, peptide sequence VSGWALFKKIS [107], synthesized by Genscript), and trypsin (Sigma #T1426;

(16) A group B streptococcal type VII-secreted LXG toxin mediates interbacterial competition and colonization of the murine female genital tractmBioOctober 16, 2024Alyx M. Job, Kelly S. Doran, Brady L. Spencerdetected in each fraction using the Nano-Glo HiBiT Extracellular Detection System (Promega Catalog# N2420) according to the manufacturer’s instructions. Luminescence was measured with a Tecan Infinite M Plex

(17) Alzheimer’s disease linked Aβ42 exerts product feedback inhibition on γ-secretase impairing downstream cell signalingeLifeJuly 19, 2024Katarzyna Marta Zoltowska, Utpal Das, Sam Lismont, Thomas Enzlein, Masato Maesako, Mei C Q Houser, Maria Luisa Franco, Burcu Özcan, Diana Gomes Moreira, Dmitry Karachentsev, et al.and 02092242T." Commercial assay or kit,Nano-Glo HiBiT Extracellular Detection System,Promega,Cat# N2420, Commercial assay or kit,LDH-Glo Cytotoxicity Assay,Promega,Cat#J2380, Commercial assay or kit,CellTiter-Glo 2.0 Assay

(18) A PRMT5-ZNF326 axis mediates innate immune activation upon replication stressScience AdvancesJune 7, 2024Phuong Mai Hoang, Denis Torre, Patrick Jaynes, Jessica Ho, Kevin Mohammed, Erik Alvstad, Wan Yee Lam, Vartika Khanchandani, Jie Min Lee, Chin Min Clarissa Toh, et al.with HiBiT extracellular detection at 1:1 ratio according to the manufacturer’s protocol (Promega, #N2420). The remaining cells in 50-μl culture medium were subjected to CellTiter-Glo (CTG) detection for viability

(19) Developing a platform for secretion of biomolecules in Mycoplasma feriruminatorisMicrobial Cell FactoriesApril 30, 2024Javier Gonzalez-de-Miguel, Ariadna Montero-Blay, Ludovica Ciampi, Irene Rodriguez-Arce, Luis SerranoThe presence of Isunakinra was checked using the Extracellular HiBit quantification assay (Promega, N2420) at a reduced volume following the instructions of the manufacturer. To do that, 10 µl sample was mixed

(20) A New Derivative of Retro-2 Displays Antiviral Activity against Respiratory Syncytial VirusInternational Journal of Molecular SciencesDecember 28, 2023Adrien Le Rouzic, Jenna Fix, Robin Vinck, Sandrine Kappler-Gratias, Romain Volmer, Franck Gallardo, Jean-François Eléouët, Mathilde Keck, Jean-Christophe Cintrat, Julien Barbier, et al.Nano-Glo HiBiT Lytic Detection System (Promega, N3030) or the Nano-Glo HiBiT Extracellular Detection System (Promega, N2420), respectively, according to manufacturer’s protocol. For each experiment, cells were finally lysed

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Nano-Glo HiBiT Extracellular Detection System

Nano-Glo HiBiT Extracellular Detection System from Promega

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Nano-Glo HiBiT Extracellular Detection System from Promega

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