Description
Cellecta’s NGS Prep Kit for sgRNA Libraries cloned into the LentiGuide vector (e.g., GeCKO, Brunello) provides a protocol and reagents for the PCR amplification from genomic DNA and NGS sequencing of sgRNA sequence cassette included in these libraries. Sufficient reagents are provided to process 12-48 DNA samples (depending on the amount of DNA/sample) with unique indexing primers to multiplex up to 12 samples run in a single lane. Software provided with the Kit enable demultiplexing of the FASTQ files