Fig 1: NRs induce lipid absorption-related genes at both the mRNA and protein level in enterocytes. (A) Expression of PLIN2 across different nuclear receptor agonists. n = 2 with 3 donors. (B) Representative max projection images of PLIN2 stainings at 10× (Scale bar, 400 μm) and at 63x in the insert (Scale bar, 100 μm). n = 2 with 3 donors (C) Quantification of PLIN2 stainings, based on approximately 80 organoids per condition, imaged at 10× magnification. n = 2 with 3 donors. (D) Expression of APOA4 across different nuclear receptor agonists. n = 2 with 3 donors. (E) APOA4 ELISA for donor N39. (F) Expression of APOA1 across different nuclear receptor agonists. n = 2 with 3 donors. (G) Representative max projection images of APOA1 stainings at 10× (Scale bar, 400 μm) and at 63× in the insert (Scale bar, 100 μm). n = 2 with 3 donors (H) Quantification of APOA1 stainings, based on approximately 40 organoids per condition, imaged at 10× magnification. n = 2 with 3 donors. (I) Quantification of intracellular lipid contents, based on approximately 60 organoids per condition, imaged at 10× magnification. n = 2 with 3 donors. (J) Representative confocal images of intracellular lipid contents, stained with Nile red. Organoids were imaged at 10× (Scale bar, 400 μm) and 63x in the inserts (Scale bar, 100 μm) (inserts). n = 2 with 3 donors. Error bars =SD, *P < 0.05, **P < 0.005, ***P < 0.0005 by one-way ANOVA of each NR condition compared to the control or t test compared to the control.