Fig 1: Effect of salusin-β knockdown on LPS-induced release of inflammatory cytokines. (A) Protein expression of salusin-β in NR8383 cells transfected with shRNA-salusin-β-1/2 or sh-NC (n=3). (B) mRNA expression of salusin-β in NR8383 cells transfected with shRNA-salusin-β-1/2 or sh-NC (n=3). (C) Concentrations of TNF-α, IL-1β, IL-6 and MCP-1 in the culture medium of LPS-induced NR8383 cells with or without salusin-β knockdown (n=3). ***P<0.001 vs. control; ###P<0.001 vs. sh-NC. NC, negative control; LPS, lipopolysaccharide; MCP-1, monocyte chemotactic protein 1; shRNA, short hairpin RNA.
Fig 2: Inhibitory effect of ARF6 knockdown on the inflammatory response and oxidative stress injury in HG-treated H9c2 cells. Following 2 h of 33 mmol/l glucose treatment, H9c2 cells were transfected with pcDNA3.1-ARF6, sh-ARF6 or corresponding negative controls, and the transfection efficiency was examined by (A) reverse transcription-quantitative PCR and (B) western blotting. Levels of (C) IL-6, (D) TNF-a and (E) MCP-1 were assessed by ELISA. (F) LDH content was determined via LDH assays. (G) ROS content was measured by ROS kits. (H) MDA content and (I) activities of antioxidant enzymes were evaluated by ELISA. (J) Detection of H9c2 cell apoptotic rate by flow cytometry. *P<0.05, **P<0.01 and ***P<0.001 vs. indicated groups. ARF6, ADP ribosylation factor 6; HG, high glucose; sh, short hairpin; MCP-1, monocyte chemoattractant protein 1; LDH, lactate dehydrogenase; ROS, reactive oxygen species; MDA, malondialdehyde; NC, negative control.
Fig 3: Effects of anethole (125 and 250 mg/kg) administration for 14 days prior to renal ischemia perfusion (RIR) on the renal inflammatory mediators (a) TNF-α, (b) IFN-γ, (c) MCP-1, and (d) IL-10, and on apoptosis, including (e) caspase 3 and (f) caspase 9, in RIR-induced injury. All values are stated as mean ± SD. # designates statistically significant compared to sham group, * designates statistically significant compared to RIR group, and @ designates statistically significant compared to RIR + anethole 125 mg/kg group (p < 0.05) using one-way ANOVA followed by Tukey’s post hoc test.
Fig 4: The influences of geraniol (100 and 200 mg/kg) administration for 14 days prior to renal ischemia/perfusion (I/R) induced injury on the renal inflammatory mediators (a) TNF-a, (b) IFN-?, (c) MCP-1, and (d) IL-10, and on apoptosis including gene (mRNA) expression of (e) Bax, (f) Bcl-2 and (g) Caspase 3, and (h) Caspase 9. All values are expressed as mean ± SD. ¥ indicates statistically significant from the sham group, ? indicates statistically significant from the renal I/R group, and f indicates statistically significant from renal I/R + geraniol 100 mg/kg group (p < 0.05) using one-way ANOVA followed by Tukey’s post hoc test.
Fig 5: Salusin-ß is upregulated in LPS-induced acute lung injury. (A) Representative hematoxylin and eosin staining of lung tissues of rats in the LPS or control group. (B) Concentrations of TNF-a, IL-1ß, IL-6 and MCP-1 in the bronchoalveolar lavage fluid of rats in the LPS or control group (n=5). (C) Protein expression of salusin-ß and CD68 in lung tissues of rats in the LPS or control group (n=5). (D) Protein expression of salusin-ß in NR8383 cells treated with or without 1 µg/ml LPS for 6, 12, 24 and 48 h (n=3). **P<0.01 and ***P<0.001 vs. control. LPS, lipopolysaccharide; MCP-1, monocyte chemotactic protein 1.
Supplier Page from Abcam for Rat MCP1 ELISA Kit