Fig 1: The serum level of HMGB1, IL-6, IL8, and TNF-a in HD group and PD group. HD = hemodialysis; HMGB1 = high mobility group box protein-1; IL = interleukin; PD = peritoneal dialysis.
Fig 2: Cholesterol depletion hyper-activates LTßR-dependent pro-inflammatory responsea, b The concentrations of secreted CXCL8 were measured with ELISA in media collected from cells preincubated for 1 h with MßCD and then stimulated or not for 4 h (a) or 8 h (b) with Ago or LTa1ß2 in the presence or absence of MßCD. Data represent the means ± SEM, n = 4. *P = 0.05; **P = 0.01 by Mann-Whitney or Student’s t-test. c Lysates of A549 cells pretreated for 1 h with MßCD and then stimulated or not for 8 h with LTa1ß2 or Ago in the presence or absence of MßCD were analyzed by Western blotting with antibodies against the indicated proteins. Vinculin was used as a loading control. Graph shows densitometric analysis for ICAM1 from Western blotting (protein levels normalized to vinculin). Values are presented as fold change versus controls - unstimulated and untreated cells (black bars). Data represent the means ± SEM, n = 4; ns - P > 0.05; *P = 0.05; **P = 0.01 by one sample t-test (in grey) or by Mann-Whitney (in black). d, f Adhesion of Jurkat, NK cells, neutrophils and T lymphocytes to A549 cells (d) and HUVECs (f) treated as in a or e, respectively. Graphs represent quantification of immune cell adhesion to A549 and HUVECs relative to control (untreated) cells. Values are presented as fold change versus controls - unstimulated and untreated cells (black bars). Data represent the means ± SEM, n = 3 (d), n = 3 (f); ns - P > 0.05; *P = 0.05; **P = 0.01 by one sample t-test. e Lysates of HUVECs preincubated for 1 h with MßCD and then stimulated or not for 6 h with LTa1ß2 in the presence or absence of MßCD were analyzed by Western blotting with antibodies against the indicated proteins. Vinculin was used as a loading control.
Supplier Page from Abcam for Human IL-8 ELISA Kit