Fig 1: DJ-1 protein in maize mitochondria and plastids. The amount of DJ-1 protein from isolated mitochondria and plastids was measured using anti-DJ-1 antibodies with competitive ELISA (A–D) and slot blot (E–H) assays. For ELISA, DJ-1 protein in mitochondria was measured as ng/mL. Representative images of slot blot assays are shown (I–L) and the relative DJ-1 protein was determined, where the image intensity was set to 1 for leaf 1 (L1) in (E, G) and for light-grown leaves in (F, H). Uppermost panels of I-L show DJ-1 proteins, middle panels show the total protein (TP) levels in samples, and lowermost panels show mitochondrial (COX2)- and plastid (RbcL)-specific proteins (COX2 in panels I and J; RbcL in panels K and L). Cross-reactivity of maize organellar DJ-1 with anti-DJ-1 antibodies (human Park7) was confirmed and is shown in Supplementary Information, Fig. S1.