Fig 1: lncRNA HAND2-AS1 downregulated ROCK1 expression in HPV-positive and negative CSCC cells but not in normal cells. (A) lncRNA HAND2-AS1 overexpression led to downregulated ROCK1 expression in C-33A and SiHa human CSCC cell lines but not in HCvEpC (HPV negative) and Ect1/E6E7 (HPV positive) human cervical epithelial cell lines. (B) ROCK1 overexpression resulted in no significant effects on lncRNA HAND2-AS1 expression in all 4 cell lines. *P<0.05. lncRNA, long non-coding RNA; HAND2-AS1, HAND2 antisense RNA 1; ROCK1, rho associated coiled-coil containing protein kinase 1; HPV, human papillomavirus; CSCC, cervical squamous cell carcinoma; C, control; NC, negative control.
Fig 2: HAND2-AS1 is downregulated and ROCK1 is upregulated in HPV-positive and negative patients with CSCC compared with healthy controls. (A) HAND2-AS1 was downregulated and (B) ROCK1 was upregulated in HPV-positive and -negative patients with cervical squamous cell carcinoma compared with healthy controls. *P<0.05. HAND2-AS1, HAND2 antisense RNA 1; ROCK1, rho associated coiled-coil containing protein kinase 1; HPV, human papillomavirus.
Fig 3: Circ_0057558 promoted lipogenesis via targeting miR-206/ROCK1 axis.A Representative oil-red o staining images of transfected circ_0057558 overexpressing vector cells following FFA or control treatment. B Secreted TG levels in transfected circ_0057558 overexpressing vector cells following FFA or control treatment. C Protein levels of lipogenesis-related proteins in transfected circ_0057558 overexpressing vector cells following FFA or control treatment. D Quantifications of C. *p < 0.05, **p < 0.01, and ***p < 0.001.
Fig 4: miR-206 inhibited lipogenesis and TG secretion in vivo.A Representative images of H&E staining (upper), oil-red o staining (middle), and IHC staining (bottom) in liver tissues from HFD-fed mice or control mice with injection of miR-206 mimics or NC. B Secreted TG levels in liver tissues from HFD-fed mice or control mice with injection of miR-206 mimics or NC. C miR-206 levels in liver tissues from HFD-fed mice or control mice with injection of miR-206 mimics or NC. D ROCK1 mRNA levels in liver tissues from HFD-fed mice or control mice with injection of miR-206 mimics or NC. E, F ROCK1/AMPK activities in liver tissues from HFD-fed mice or control mice with injection of miR-206 mimics or NC. G Protein levels of ROCK1, p-AMPK, and lipogenesis-related proteins in liver tissues from HFD-fed mice or control mice with injection of miR-206 mimics or NC. H Quantifications of G. *p < 0.05, **p < 0.01, and ***p < 0.001.
Fig 5: miR-206 inhibited lipogenesis via ROCK1.A Representative oil-red o staining images of cells with the transfection of miR-206 inhibitor or NC together with sh-ROCK1 or sh-NC following FFA or control treatment. B Secreted TG levels in cells transfected with miR-206 inhibitor or NC together with sh-ROCK1 or sh-NC following FFA or control treatment. C Protein levels of lipogenesis-related proteins in cells with the transfection of miR-206 inhibitor or NC together with sh-ROCK1 or sh-NC following FFA or control treatment. D Quantifications of C. *p < 0.05, **p < 0.01, and ***p < 0.001.
Supplier Page from Abcam for ROCK Activity Assay Kit