DNA Damage Assay Kit (AP sites, Colorimetric) ab211154 from Abcam

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ItemDNA Damage Assay Kit (AP sites, Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab211154
Quantity50 Test
Sample TypeDNA

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(1) Exploring multiorgan mitochondrial dysfunction in the switch toward progressive MASLD in AMLN miceiScienceSeptember 19, 2025Meroni M, Paolini E, Longo M, Battistin M, Dondossola D, Ripolone M, Napoli L, Mosca E, Corti S, Dongiovanni P.of purified genomic DNA extracted from liver samples by using the DNA damage – AP sites – Assay Kit (ab211154, Abcam, Cambridge, UK). Conversely, mitochondrial DNA damage induced by ROS was revealed by measuring

(2) Loss of proton-sensing GPR4 reduces tumor progression in mouse models of colon cancerMolecular oncologyMay 21, 2025Perren L, Busch M, Ruiz PA, Malagola E, Baumeler V, Foti F, Gross A, Grütter T, Edel H, Schuler C, et al.E + I). 2.4 Quantification of AP sites DNA damage was determined using a colorimetric assay kit (ab211154; Abcam, Cambridge, UK) according to the manufacturer's instructions. Briefly, snap‐frozen whole colon

(3) Subcytotoxic transepidermal delivery using low intensity cold atmospheric plasmaSci RepJanuary 16, 2025Ga Ram Ahn, Hyung-Joon Park, Yu Jin Kim, Min Gyo Song, Hye Sung Han, Woo Geon Lee, Hyuck Ki Hong, Kwang Ho Yoo, Joon Seok, Kyu Back Lee, et al.8-hydroxy-2-deoxyguanosine enzyme-linked immunosorbent assay (ELISA) kit measured RS-induced DNA damage (ab211154, Abcam, Cambridge, England). The plate was read at 450 nm and the sample absorbance was compared with

(4) Unprocessed genomic uracil as a source of DNA replication stress in cancer cellsMolecular cellJune 6, 2024Saxena S, Nabel CS, Seay TW, Patel PS, Kawale AS, Crosby CR, Tigro H, Oh E, Vander Heiden MG, Hata AN, et al.tested in U2OS cells 48 h after transfection with control and UNG siRNA by a DNA damage assay kit (ab211154, Abcam) according to the manufacturer’s instructions. U-comet assay The comet assay was performed

(5) Novel anti-Acanthamoeba effects elicited by a repurposed poly (ADP-ribose) polymerase inhibitor AZ9482Frontiers in Cellular and Infection MicrobiologyMay 28, 2024Lijun Chen, Wei Han, Wenwen Jing, Meng Feng, Qingtong Zhou, Xunjia ChengTrophozoite DNA damage was assessed using the DNA Damage-AP Sites-Assay Kit (AP sites, Colorimetric; Abcam, ab211154; Cambridge, UK). DNA was diluted to 100 μg/mL in TE buffer [10 mM Tris (pH 7.5) and 1 mM EDTA], and

(6) Beneficial Effects of Fibroblast Growth Factor-1 on Retinal Pigment Epithelial Cells Exposed to High Glucose-Induced Damage: Alleviation of Oxidative Stress, Endoplasmic Reticulum Stress, and Enhancement of AutophagyInternational journal of molecular sciencesMarch 11, 2024Huang HW, Yang CM, Yang CH.USA). The quantification of AP sites in the isolated DNA was performed with the DNA Damage Assay Kit (ab211154; Abcam, Cambridge, MA, USA) following the manufacturer’s instructions. This colorimetric assay measures

(7) Profiling the metabolome of uterine fluid for early detection of ovarian cancerCell Reports MedicineJune 20, 2023Pan Wang, Jihong Ma, Wenjing Li, Qilong Wang, Yinan Xiao, Yuening Jiang, Xiaoyang Gu, Yu Wu, Suwei Dong, Hongyan Guo, et al.kit,Elabscience,Cat#E-EL-0132c Reactive Oxygen Species Detection Assay Kit,Abcam,Cat#ab186027 AP sites assay kit,Abcam,Cat#ab211154 DNeasy Blood & Tissue Kit,Qiagen,Cat#69504 ,, Deposited Data,Deposited Data,Deposited Data ,, Results

(8) Can Maternal Exposure to Air Pollution Affect Post-Natal Liver Development?ToxicsJanuary 9, 2023Yong Song, Ling Chen, Ellen Bennett, Amanda J. Wheeler, Katherine Southam, Seiha Yen, Fay Johnston, Graeme R. Zoskyusing a Genomic DNA Isolation Kit (ab65358, Abcam, Melbourne, Australia), while a DNA damage Assay Kit (ab211154, Abcam, Melbourne, Australia) was used to detect AP sites. 2.6. RNA Isolation and qPCR Total RNA was

(9) Hepatic Mitochondrial Dysfunction and Risk of Liver Disease in an Ovine Model of “PCOS Males”BiomedicinesMay 31, 2022Katarzyna J. Siemienowicz, Panagiotis Filis, Jennifer Thomas, Paul A. Fowler, W. Colin Duncan, Mick T. RaeHepatic DNA Damage Determination Hepatic DNA damage was assessed by using DNA damage-AP sites-Assay Kit (ab211154, Abcam, Cambridge, UK). DNA was diluted to 50 μg/mL in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA), and

(10) Comparative Toxicogenomics of Glyphosate and Roundup Herbicides by Mammalian Stem Cell-Based Genotoxicity Assays and Molecular Profiling in Sprague-Dawley RatsToxicological SciencesFebruary 28, 2022Robin Mesnage et al.caused by oxidative damage. This was done using the DNA Damage Assay Kit (AP sites, Colorimetric; Abcam, ab211154; Abcam plc, Cambridge, UK) according to manufacturer’s instructions. Real-time quantitative PCR We

(11) Multi-omics phenotyping of the gut-liver axis reveals metabolic perturbations from a low-dose pesticide mixture in ratsCommunications BiologyDecember 1, 2021Robin Mesnage et al.lesions caused by oxidative damage. We used the DNA Damage Assay Kit (AP sites, Colorimetric; Abcam, ab211154; Abcam plc, Cambridge, UK), according to manufacturer’s instructions. Statistics and reproducibility The

(12) TRIB2 desensitizes ferroptosis via βTrCP-mediated TFRC ubiquitiantion in liver cancer cellsCell Death DiscoveryJuly 27, 2021Susu Guo, Yuxin Chen, Xiangfei Xue, Yueyue Yang, Yikun Wang, Shiyu Qiu, Jiangtao Cui, Xiao Zhang, Lifang Ma, Yongxia Qiao, et al.and DNA damage were tested by a cell counting kit-8 (CCK-8, HY-K0301, MCE) and DNA damage assay kit (ab211154, Abcam), respectively, according to the manufacturer’s instructions. Measurement of reduced GSH GSH

(13) Mild phenotype of knockouts of the major apurinic/apyrimidinic endonuclease APEX1 in a non-cancer human cell linePloS oneJanuary 1, 2021Kim DV, Kulishova LM, Torgasheva NA, Melentyev VS, Dianov GL, Medvedev SP, Zakian SM, Zharkov DO.control. Aldehyde reactive probe assay The levels of AP sites were determined using the DNA damage assay kit (ab211154, Abcam, Cambridge, UK) according to the manufacturer’s protocol. Cells were seeded in 6-well plates

(14) MacroH2A1 Regulation of Poly(ADP-Ribose) Synthesis and Stability Prevents Necrosis and Promotes DNA RepairMolecular and cellular biologyDecember 11, 2019Ruiz PD, Hamilton GA, Park JW, Gamble MJ.for 1 h at 37°C. Colorimetric measurement of AP sites was performed using a commercial kit (Abcam; ab211154) following the manufacturer’s protocol. The optical density at 450 nm was normalized using the standard

(15) Mutation in DNA Polymerase Beta Causes Spontaneous Chromosomal Instability and Inflammation-Associated Carcinogenesis in MiceCancers (Basel)August 13, 2019Shengyuan Zhao, Alex W. Klattenhoff, Megha Thakur, Manu Sebastian, Dawit Kidanestandard DNA solutions as described previously (DNA Damage AP sites assay kit, Colorimetric, Cat # ab211154; Abcam). 3.5. Immunofluorescence Localization Cover slips (Thermo FIsher, Waltham, MA, USA, 154526)

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DNA Damage Assay Kit (AP sites, Colorimetric) from Abcam

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DNA Damage Assay Kit (AP sites, Colorimetric) from Abcam

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