Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit ab214663 from Abcam

Product Specs
ItemAnnexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab214663
Quantity100 Test
TypeStain
Gene NameANXA5
Sample TypeAdherent cells, Suspension cells

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Bypass of LANA-DNA crosslinks by Polη ensures KSHV genome maintenance and tumor growthCell ReportsJune 23, 2026Jayaraju Dheekollu, Coltin J. Albitz, Anjali Pathania, Frederick Keeney, Samantha S. Soldan, Paul M. Liebermanevaluation of apoptosis as per the protocols described earlier using an annexin V and 7-AAD-based kit (#ab214663, Abcam, Melbourne, VIC, Australia). For PFGE studies cells were treated with either vehicle control

(2) WWP1 gain-of-function drives developmental anoikis through TGFβ pathway during neurodevelopmentCell Death DiscoveryMarch 6, 2026Ki Hurn So, Seungbok Lee, Jiayi Wong, Hyunsik Lee, Eun-Jin Yun, Se Song Jang, Hee-Jung Choi, Jong-Hee Chae, Seung Tae Baeklevel was evaluated using the Annexin V-CF Blue/7-Aminoactinomycin D (7-AAD) Apoptosis Detection Kit (ab214663, Abcam) according to the manufacturer’s protocols. Briefly, adherent cells were gently detached using

(3) Postbiotics Combination Synergises the Antiproliferative Effects of Doxorubicin in Gastric Cancer Cells: A Cellular and Molecular Deep DiveInternational Journal of Molecular SciencesDecember 29, 2025Radwa A. Eladwy, Mohamed Fares, Muhammad A. Alsherbiny, Dennis Chang, Chun-Guang Li, Deep Jyoti BhuyanAGS adenocarcinoma cells were evaluated using an annexin V and 7-AAD-based apoptosis detection kit (#ab214663, Abcam, Melbourne, VIC, Australia) following established protocols [33,163]. Briefly, AGS gastric cancer

(4) Combination therapy with nisin, urolithin B, and vincristine exhibits synergistic antiproliferative and pro-apoptotic effects against human lymphoma cells: evidence from proteomicsFrontiers in ImmunologySeptember 23, 2025Ahmad K. Al-Khazaleh, Muhammad A. Alsherbiny, Dennis Chang, Gerald Münch, Deep Jyoti Bhuyanthe HKB-11 lymphoma cells after 24 h treatment was studied using an annexin V and 7-AAD-based kit (#ab214663, Abcam, Melbourne, VIC, Australia) (9, 14, 15, 22). The HKB-11 cells were cultured in T75 cell culture

(5) Investigating the Cellular Responses to Combined Nisin and Urolithin B Treatment (7:3) in HKB-11 Lymphoma CellsInternational Journal of Molecular SciencesJuly 30, 2025Ahmad K. Al-Khazaleh, Muhammad A. Alsherbiny, Dennis Chang, Gerald Münch, Deep Jyoti Bhuyanthe HKB-11 lymphoma cells after 24 h treatment were studied using an annexin V and 7-AAD-based kit (#ab214663, Abcam, Melbourne, VIC, Australia) [9,81]. The HKB-11 cells were cultured in T75 cell culture flasks

(6) Fuelling the Fight from the Gut: Short-Chain Fatty Acids and Dexamethasone Synergise to Suppress Gastric Cancer CellsCancers (Basel)July 28, 2025Radwa A. Eladwy, Mohamed Fares, Dennis Chang, Muhammad A. Alsherbiny, Chun-Guang Li, Deep Jyoti Bhuyanµg/mL), on apoptosis in AGS gastric adenocarcinoma cells was assessed using an annexin V/7-AAD kit (#ab214663, Abcam) as previously described [20,21]. Briefly, AGS cells (1 × 106 cells/10 mL) were cultured in T75

(7) Gut Microbial Postbiotics as Potential Therapeutics for Lymphoma: Proteomics Insights of the Synergistic Effects of Nisin and Urolithin B Against Human Lymphoma CellsInt J Mol SciJuly 16, 2025Ahmad K. Al-Khazaleh, Muhammad A. Alsherbiny, Gerald Münch, Dennis Chang, Deep Jyoti Bhuyanthe HKB-11 lymphoma cells after 24 h treatment was studied using an Annexin V- and 7-AAD-based kit (#ab214663, Abcam, Melbourne, VIC, Australia) [25,152]. The HKB-11 cells were cultured in T75 cell culture flasks

(8) AVJ16 inhibits lung carcinoma by targeting IGF2BP1OncogeneJuly 8, 2025Nadav Wallis, Tehila Gershon, Sojod Shaaby, Froma Oberman, Myriam Grunewald, Deborah Duran, Amandeep Singh, Gilad Vainer, Vladimir S. Spiegelman, Arun K. Sharma, et al.performed using FACS using Annexin V–CF Blue/7-amino-actinomycin D (7-AAD) Apoptosis Detection Kit (ab214663, Abcam) according to the manufacturer’s protocol. Briefly, cells were detached using 0.05% trypsin and

(9) Short- and Long-Term Endothelial Inflammation Have Distinct Effects and Overlap with Signatures of Cellular SenescenceCellsMay 30, 2025Barbora Belakova, José Basílio, Manuel Campos-Medina, Anna F. P. Sommer, Adrianna Gielecińska, Ulrike Resch, Johannes A. Schmidfollowed by an additional washing step with PBS. Cells were subsequently incubated in 5 µg/mL 7-AAD (ab214663, Abcam) and 100 µg/mL RNase A for 30 min, shielded from light. Incorporation of 7AAD into DNA was quantified

(10) Restricting SLC7A5-mediated Leucine uptake in T cells prevents acute GVHD and maintains GVT responseEMBO Molecular MedicineMay 21, 2025Nieves Fernández-Gallego, Blanca Anega, Susana Luengo-Arias, Maider Bizkarguenaga, Rubén Gil-Redondo, Nieves Embade, Laura Navarrete-Arias, Marta Ramírez-Huesca, Emigdio Álvarez-Corrales, Sara G Dosil, et al.diet),"Research Diets, Inc",A05080202i Annexin V-CF Blue 7-AAD Apoptosis Staining/Detection Kit,Abcam,ab214663 Antimycin A,Sigma-Aldrich,A8674-50MG BAM15,Sigma-Aldrich,SML1760-5MG Brefeldin A,Sigma-Aldrich,B7651-5MG CellTrace Violet Cell Proliferation Kit

(11) Enhancement of Autophagy in Macrophages via the p120-Catenin-Mediated mTOR Signaling PathwayThe Journal of ImmunologyDecember 1, 2024Suganya Kanmani, Xue-Min Song, Paulraj Kanmani, Xiao-Jing Wu, Xiao-Di Xiao-Di-Tan, Jing Liu, Ji-Ping Wang, Richard D. Minshall, Guochang Huharvested to analyze the apoptotic cells using Annexin V-CF Blue/7-AAD apoptosis detection kit (Abcam, ab214663,), according to the manufacturer protocol. The stained cells were analyzed by flow cytometry (CytoFelx,

(12) Effects of Chronic Inflammatory Activation of Murine and Human Arterial Endothelial Cells at Normal Lipoprotein and Cholesterol Levels In Vivo and In VitroCellsApril 30, 2024Mussbacher M, Basílio J, Belakova B, Pirabe A, Ableitner E, Campos-Medina M, Schmid JA.and incubated with Annexin V-CF Blue and 7-AAD staining solutions using an Apoptosis Detection Kit (ab214663, Abcam, Cambridge, UK) according to the manufacturer’s recommendations. Stained cell suspensions were

(13) The postbiotic sodium butyrate synergizes the antiproliferative effects of dexamethasone against the AGS gastric adenocarcinoma cellsFront NutrMarch 12, 2024Radwa A Eladwy, Muhammad A. Alsherbiny, Dennis Chang, Mohamed Fares, Chun-Guang Li, Deep Jyoti Bhuyanapoptosis profiles of the AGS adenocarcinoma cells was studied using an annexin V and 7-AAD-based kit (#ab214663, Abcam, Melbourne, VIC, Australia) (19, 29). The AGS cells were cultured in T75 cell culture flasks

(14) Mechanistic Insights into the Anti-Proliferative Action of Gut Microbial Metabolites against Breast Adenocarcinoma CellsInternational Journal of Molecular SciencesOctober 10, 2023Kayla Jaye, Muhammad A. Alsherbiny, Dennis Chang, Chun-Guang Li, Deep Jyoti Bhuyanbutyrate, nisin and inosine as per the protocols described earlier using an annexin V and 7-AAD-based kit (#ab214663, Abcam, Melbourne, VIC, Australia) [40,71]. Annexin V detects phosphatidylserine on the external membrane

(15) Application of Gold Nanoparticles as Radiosensitizer for Metastatic Prostate Cancer Cell LinesInternational Journal of Molecular SciencesFebruary 18, 2023Sílvia Soares, Isabel Faria, Fátima Aires, Armanda Monteiro, Gabriela Pinto, Maria Goreti Sales, Miguel A. Correa-Duarte, Susana G. Guerreiro, Rúben Fernandesobtained from Invitrogen Co. (Scotland, UK); Annexin V-CF Blue 7-AAD apoptosis staining/detection kit (ab214663) from Abcam; DCFDA/H2DCFDA—Cellular ROS Assay Kit (D399) from Invitrogen; and QIAzol from Qiagen. 4.2.

(16) Impact of latency-reversing agents on human macrophage physiologyImmunity, inflammation and diseaseJanuary 1, 2023Hany L, Turmel MO, Barat C, Ouellet M, Tremblay MJ.all purchased from ThermoFisher Scientific. Annexin V‐CF Blue 7‐AAD Apoptosis Staining/Detection Kit (ab214663) was purchased from Abcam (Cambridge, United Kingdom). QUANTI‐Blue™ (rep‐qb1) was obtainned from Invivogen.

(17) Targeting DNA repair to enhance the efficacy of sorafenib in hepatocellular carcinomaJournal of Cellular BiochemistryOctober 22, 2022Mahzeiar Samadaei, Daniel Senfter, Sibylle Madlener, Karolina Uranowska, Christine Hafner, Michael Trauner, Nataliya Rohr‐Udilova, Matthias Pinter10.6.1 (TreeStar Inc.). 2.8 Apoptosis assay Annexin V‐CF Blue/7‐AAD Apoptosis Detection Kit (cat. no. ab214663; Abcam) was used to evaluate the percentage of live, apoptotic or necrotic cells. The analysis was performed

(18) Cysteine is a limiting factor for glioma proliferation and survivalMolecular oncologyMay 1, 2022Ruiz-Rodado V, Dowdy T, Lita A, Kramp T, Zhang M, Jung J, Dios-Esponera A, Zhang L, Herold-Mende CC, Camphausen K, et al.Apoptosis assay Experiments were performed using the 7AAD Annexin V Apoptosis Detection Kit (Abcam; ab214663) according to the vendor’s protocol and analyzed with a FACSCalibur (Becton Dickinson, Franklin Lakes,

(19) Progesterone Attenuates SIRT1-Deficiency-Mediated Pre-EclampsiaBiomoleculesMarch 9, 2022Jiangnan Pei et al.Apoptosis Assay HTR-8/SVneo cells from P4 and vehicle groups were stained with Annexin V and 7-AAD (ab214663, Abcam, UK) for 15 min at RT in the dark. Fluorescence intensities were analyzed by flow cytometry by

(20) K-means quantization for a web-based open-source flow cytometry analysis platformScientific ReportsDecember 1, 2021Nathan Wong, Daehwan Kim, Zachery Robinson, Connie Huang, Irina M. Conboydilution for later passaging. The apoptotic assay of IMR90 was conducted by Apoptosis Detection Kit (ab214663, Abcam) according to the manufacturer’s protocol. Briefly, cells were detached using 0.05% trypsin and

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Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit from Abcam

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Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit from Abcam

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