Fig 1: Successful generation of Fn gene conditional knockout mice.(A, B) Identification and linearization of the Fn1 gene targeting vector, and the results of Hind III digestion, the theoretical band size is 9.8 kb, 5.8 kb and 810bp (810 bp band cannot be displayed because the product is too small). (C-E) Electrophoresis map of 5' homology arm (5' arm) and 3' homology arm (3' arm) PCR identification of F1 mice (recombinant negative can only clone a single fragment). (F) The PCR product of the genome inserted into LoxP was 1.4 kb, the PCR product of KO was 0.7 kb, and the PCR product of WT mouse was 1.2 kb, electrophoresis grayscale graph showed that knockout efficiency of DNA was 99% in liver. (G) ELISA and (H, I) Western blotting results showing FN levels in the plasma and liver of Fn KO mice at different durations post tamoxifen induction, the knockout efficiency was 95%. (J-M) FN immunohistochemical and (N-Q) immunofluorescence staining of liver and kidney indicated that FN expression was decreased significantly in Fn KO mice.
Fig 2: Target vector plasmid map.Fn1 target vector plasmid map.
Supplier Page from Abcam for Mouse Fibronectin ELISA Kit