Fig 1: Tube formation of human endothelial cells treated by melanoma cell conditioned-media with and without GGLE treatment. Human umbilical vein endothelial cells (HUVEC) within 10 passages were seeded into Extracellular Matrix Gel and treated with conditioned medium as indicated. Tube formation was examined under fluorescent microscope after 18 hours. (A) Representative images. (B) Total number of tubes formed per well. HUVEC medium indicates medium used for HUVEC culture with all supplements and growth factors indicated in Materials and Methods. Melanoma medium indicates DMEM medium without FBS. Ang, angiogenin 50 ng/mL. Suramin (30 µM) was an inhibitor control. All GGLE treatments indicate conditioned DMEM medium of SK-Mel-2 cells treated with GGLE. Hy, hypoxia. *P < .05; **, P < .01 by one-way ANOVA with Bonferroni and Holm Multiple Comparison with pairs relative to control group simultaneously compared.