EdU Assay / EdU Staining Proliferation Kit (iFluor 488) ab219801 from Abcam

Product Specs
ItemEdU Assay / EdU Staining Proliferation Kit (iFluor 488)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab219801
Quantity50 Test
Sample TypeAdherent cells, Suspension cells
TypeStain

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Aberrant chromatin remodeling influences human neural cell fate change in Trisomy 21bioRxivJune 2, 2026Jenny A. Klein, Suraj Upadhya, Anna Nathanson, Dana Silvian, Eric F. Zaniewski, Robert Morris, Wilhelm Haas, Lindy E. BarrettThermoFisher). To mark cells in S-phase, we used the EdU Assay / EdU Staining Proliferation Kit (Abcam, ab219801). NPCs were incubated with 10 μM EdU for two hours then fixed and processed for imaging according to

(2) Bifunctional Surfaces With Immobilized Antibodies and Bioactive Peptides Mediate Selective Capture and Proliferation of Endothelial Colony-Forming CellsAdvanced healthcare materialsJune 1, 2026Level HA, Campeau MA, Elkhodiry MA, Laroche G, Tanguay JF, Hoesli CA.supplemented with 0.5% FBS and cells were left to growth overnight. An EdU staining proliferation kit (Abcam; ab219801) was used as per the manufacturer's instructions. Briefly, 0.5 mL of culture medium was replaced by

(3) Comprehensive Analysis of Genomic and Phenomic Data Reveals Context-Dependent Function of A20 (TNFAIP3) in Renal Cell CarcinomaCancers (Basel)May 28, 2026Nour Abu Jayab, Burcu Yener, Reem Sami Alhamidi, Mansi Bhavsar, Alaa Muayad Altaie, Muna Abdalla Alhammadi, Vidya Bijosh Mohan, Marwa Khamis Almazrouei, Lina Sahnoon, Rola Abujabal, et al.using a 5-ethynyl-2′-deoxyuridine (EdU) incorporation assay (EdU proliferation assay kit; cat. no. ab219801; Abcam, Cambridge, UK). HEK293 and 786-O cells were seeded in 6-well plates at a density of 2 × 105

(4) Tentonin 3 regulates the proliferation and migration of neural stem cells during embryonic brain developmentBMC BiologyApril 21, 2026Maria Al Nuilati, Kyungmin Kim, Jimin Kang, Min Soo Kim, Uhtaek Oh, Gyu-Sang Hongproliferation assay The EdU Proliferation assay was performed using the EdU Proliferation Kit (Abcam, Cat. No. ab219801) according to the manufacturer’s instructions. Briefly, neurospheres were dissociated into single cells

(5) B-type lamins maintain transcriptional homeostasis by spatially controlling chromatin-speckle proximityNucleic Acids ResMarch 30, 2026Geun-Seup Shin, Jinho Kim, Ji-Young Kim, Chul-Hong Kim, Mi-Jin An, Hyun-Min Lee, Ah-Ra Jo, Yuna Park, Yujeong Hwangbo, Tae Kyung Hong, et al.reaction was performed utilizing the components included in Click it EdU-Alexa-Flour488 Imaging Kit (ab219801, Abcam). After fixation, permeabilization, and blocking of the cells, EU incorporation was visualized

(6) Unravelling the anti-cancer mechanisms elicited by non-covalent thioredoxin reductase inhibitors for triple negative breast cancer therapyRedox BiologyMarch 1, 2026Abigail Rullo, Brenna Flowers, Keacha Chang, An Zhang, Valentina Z. Petukhova, Luke Harding, Sammy Y. Aboagye, Maurizio Bocchetta, Wei Qiu, David L. Williams, et al.stained according to the manufacturer's instructions using an EdU Staining Proliferation Kit iFluor 488 (ab219801) from Abcam. Proliferation was immediately analyzed using NovoCyte Quanteon Flow Cytometer by Agilent

(7) Influence of stage-specific and location-related intratumoral microbiota on prognosis and metabolic reprogramming in non-small cell lung cancerFrontiers in ImmunologyJanuary 5, 2026Yuwei Zhao, Zhuang Tong, Lin Liu, Rongjie Yang, Zhongfeng Chen, Zheng Wangwas carried out to analyze cell proliferation. Cells were incubated with EdU Proliferation Assay Kit (ab219801, Abcam, UK) following the manufacturer’s protocol. After an incubation period of 2–4 hours, the cells

(8) The anti-cancer role of tumor protein p53-inducible nuclear protein 2/nuclear factor-kB/nuclear factor erythroid 2-related factor 2/heme oxygenase-1 pathway in spinal cord gliomaCytojournalOctober 23, 2025Jiang Huang, Zhimin Pan, Lei Yu, Xing Zhu, Yangbin Wang, Yuanguo Zhou, Kai Huang, Xingen Zhuevaluated U251 cell viability. The 5-ethynyl-2’-deoxyuridine (EdU) proliferation assay kit (cat.no. ab219801) was purchased from Abcam (Cambridge, UK). Approximately 50 μM EdU was added to U251 cells and incubated

(9) The anti-cancer role of tumor protein p53-inducible nuclear protein 2/nuclear factor-kB/nuclear factor erythroid 2-related factor 2/heme oxygenase-1 pathway in spinal cord gliomaCytojournalOctober 23, 2025Jiang Huang, Zhimin Pan, Lei Yu, Xing Zhu, Yangbin Wang, Yuanguo Zhou, Kai Huang, Xingen Zhuevaluated U251 cell viability. The 5-ethynyl-2’-deoxyuridine (EdU) proliferation assay kit (cat.no. ab219801) was purchased from Abcam (Cambridge, UK). Approximately 50 μM EdU was added to U251 cells and incubated

(10) Choroid Plexus Fibroblast–ILC2 Niche Promotes Adult Hippocampal Neurogenesis after Traumatic Brain InjuryAdvanced ScienceSeptember 1, 2025Shiqi Gao, Xiaoming Guo, Sixuan Tian, Huaping Huang, Caidi Ying, Junjie Wang, Jiahao Zhang, Jun Lin, Anwen Shao, Jingyu Wang, et al.Proliferation Assay The proliferation of NSCs was assessed with an EdU Staining Proliferation Kit (ab219801, Abcam, UK) as the previous work described.[ 61 ] NSCs that undergone the ILC2 or rAREG treatment

(11) Engineered MEVs for photoreceptor-targeted delivery of USP25 to alleviate diabetic retinopathyJ NanobiotechnologyAugust 20, 2025Yaoxiang Sun, Shenyuan Chen, Xiaoyuan Qi, Yuntong Sun, Zhengmei Jiang, Jie Chang, Yongjun Ma, Jin Huang, Benshuai You, Fengtian Sunpermeabilization with 0.1% Triton X-100 for 20 min, 661 W cells were treated with the EdU Assay Kit (ab219801, Abcam, USA) for 30 min, incubated with DAPI buffer for 15 min and photographed using a microscope. Cell

(12) DNMT1 blocks SOX21-repressed CKS2 transcription to promote gastric cancer progressionBMC CancerJuly 17, 2025Jie Wei, Song Xue, Xinglong Du, Yuanfei Dai, Yuting Ji, Guangsi HeImages were acquired and photographed using fluorescence microscopy. EdU assay The EdU staining kit (ab219801, Abcam) was used to detect cell proliferation. After 60% of the GC cells had adhered to the wall, the

(13) Piperine Enhances Mitochondrial Biogenesis to Mitigate Stress in SH-SY5Y Neuroblastoma CellsFood Sci NutrJuly 16, 2025Nongluk Saikachain, Pawitchaya Charoensawat, Oramon Tuntoolavest, Napak Vejsureeyakul, Teeranart Kunpittaya, Wanangkan Poolsri, Titiwat Sungkaworn, Rungnapha Saeeng, Chatchai Muanprasat, Nithi Asavapanumasproliferation analysis, we followed the protocol provided in the EdU Assay Kit (iFluor 488; Abcam, ab219801), using complete medium with serum as a positive control. 2.3 Mitochondrial Superoxide Measurement Mitochondrial

(14) The transcription factor IID subunit Taf13 is dispensable for TATA binding protein promoter recruitment and RNA polymerase II transcriptioniScienceJune 20, 2025Igor Martianov, Stephanie Le Gras, Guillaume Davidson, Irwin DavidsonVenor GeM Mycoplasma Detection Kit. Proliferation was measured using EdU proliferation kit (Abcam, ab219801 (iFluor 488). For spontaneous differentiation, ESCs were feeder depleted and allowed to form EBs in

(15) Genetic modeling of ELP1-associated Sonic hedgehog medulloblastoma identifies MDM2 as a selective therapeutic targetCancer CellJune 9, 2025Shiekh Tanveer Ahmad, Yiran Li, Jesus Garcia-Lopez, Brian L. Gudenas, Jennifer Hadley, Leena Paul, Stephanie C. Wu, Alaa Refaat, Marija Kojic, Melissa Batts, et al.dried slides were made and detection of EdU was done using iFluor 488 azide (provided in Abcam-Cat#ab219801, EdU proliferation kit following manufacturer’s instructions). Cells were then counter stained with

(16) Longitudinal single-cell analysis reveals RUNX1T1 as an early driver in treatment-induced neuroendocrine transdifferentiationbioRxivMay 21, 2025Yuchao Ni, Dong Lin, Mingchen Shi, Yen-Yi Lin, Hui Xue, Xin Dong, Liangliang Liu, Funda Sar, Rebecca Wu, Tunc Morova, et al.proliferation was assessed by EdU incorporation using the EdU Staining Proliferation Kit (iFluor 488) (Abcam ab219801), following the manufacturer’s instructions. Briefly, cells were pulsed with 10 μM EdU for 3 hours at

(17) Desmosome mutations impact the tumor microenvironment to promote melanoma proliferationNature GeneticsMay 1, 2025Maayan Baron, Mohita Tagore, Patrick Wall, Fan Zheng, Dalia Barkley, Itai Yanai, Jing Yang, Maija Kiuru, Richard M. White, Trey Idekerwashes. For the EdU stain, click-chemistry-based EdU detection was performed using an Abcam kit (cat. no. ab219801) according to the manufacturer’s instructions. To stain for cell-specific markers, cells were treated

(18) E2F8-induced GRPEL2 promoted colorectal cancer progression via targeting TIGARJ Transl MedApril 23, 2025Cheng Song, Lei Zhao, Jing Deng, Li Wang, Min Mao, Siyuan Peng, Wei Tangsynthesis when detecting the stability of existing proteins. Cell proliferation assay EdU assay kit (#ab219801, Abcam, Cambridge, England) was used to test cell proliferation. Cells were incubated with EdU solution

(19) Contribution of brain pericytes to neuroinflammation following repetitive head traumaJ Inflamm (Lond)March 3, 2025Arianna Cembran, Maxwell Eisenbaum, Daniel Paris, Michael Mullan, Fiona Crawford, Scott Ferguson, Corbin Bachmeierproliferation assay MBVP proliferation was measured by an Edu proliferation Kit (iFluor 488) (cat No. ab219801, Abcam). Edu solution was added to wells for 2–4 h at 37 °C with 5% CO2 prior to completion of experiment.

(20) N-Cadherin promotes cardiac regeneration by potentiating pro-mitotic β-Catenin signaling in cardiomyocytesNature communicationsJanuary 21, 2025Tsai YW, Tseng YS, Wu YS, Song WL, You MY, Hsu YC, Chen WP, Huang WH, Chng JC, Lim CL, et al.sections were used for following staining. An EdU Staining Proliferation Kit (iFluor 488 by Abcam, #ab219801) was used to stain EdU-labeled sample following the manufacturer’s instructions. In short, EdU-treated

Be the First to Write a Review!

EdU Assay / EdU Staining Proliferation Kit (iFluor 488) from Abcam

Abcam

Citations (71)

Reviews

EdU Assay / EdU Staining Proliferation Kit (iFluor 488) from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map