Fig 1: Heatmap of exosomal proteome abundance changes in disease. Heatmaps were constructed for the significantly different proteins identified in exosomes of Controls vs AD cases, isolated from serum samples of the UA-Cohort. Differences were determined using Welch’s t-test and a 95% confidence level was considered. The bars on the top of heatmaps show the kits category. Red represents higher abundance and light yellow represents lower abundance levels. Abbreviations: AACT, a-1-antichymotrypsin; APOC3, apolipoprotein C-III; APOH, beta-2-glycoprotein 1; C4BPA, C4b-binding protein alpha chain; CO3, complement C3; CO9, complement component 9; Disease, Alzheimer’s disease cases; IGHM, immunoglobulin heavy constant mu; K2C6A, keratin, type II cytoskeletal 6A; KV230, Immunoglobulin kappa variable 2–30
Fig 2: Volcano plot of significant different exosomal proteins in AD cases versus Controls. Exosomes were isolated from serum-samples of the UA-Cohort using ExoQuick. The dashed red line indicates the p-value threshold of 0.05. Abbreviations: AACT, a-1-antichymotrypsin; APOC3, apolipoprotein C-III; APOH, beta-2-glycoprotein 1; C4BPA, C4b-binding protein alpha chain; CO3, complement C3; CO9, complement component 9; Disease, Alzheimer’s disease cases; IGHM, immunoglobulin heavy constant mu; K2C6A, keratin, type II cytoskeletal 6A; KV230, immunoglobulin kappa variable 2–30
Fig 3: AACT exosomal levels in dementia and AD cases monitored by distinct antibody-based approaches. AACT levels were assessed through immunoblot analysis or commercial ELISA assays in serum-derived exosomes from Controls (CDR = 0 and MMSE -) and individuals with dementia (CDR = 1 and MMSE +) from UA-cohort (a, d), and AD clinically diagnosed cases from UA-cohort (b, e) or UMG-cohort (c, f). For WB, each point represents the relative densitometry ratio. For ELISA, each point represents the mean concentration value obtained for each individual. The solid horizontal line shows mean, and error bars indicates standard deviations. Abbreviations: AD, Alzheimer’s disease; C, Controls; CDR, Clinical Dementia Rate; MMSE, Mini-Mental State Examination. *p = 0.05
Supplier Page from Abcam for Human AACT ELISA Kit