Fig 1: ELISA and immunohistochemistry confirm gene expression data in Fig. 6. Ldlr-/- mice were fed the four diets, and the levels of protein in jejunum were determined by ELISA or the number of goblet cells or Paneth cells in jejunum were determined by immunohistochemistry as described in Materials and methods. The gender, age, and number of mice per group for each panel are shown in supplemental Table S5. A: IL-36?. B: IL-23. C: IL-22. D: NOTCH2. E: DLL4. F: ATOH1. G: GFI1. H: Goblet cells/crypt. I: Goblet cells/villus axis. J: Paneth cells/crypt. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.