Fig 1: Flow cytometry analysis of lung trNK cells markers of (A) PD-1 and (B) VDR. A multiplexed sandwich enzyme-linked immunosorbent assay-based technology was used to determine the concentration of (C) soluble PDL-1 and (D) VD3 in BALF. Data are represented as averages ± SD (n = 11 per group). Significance was determined using Newman–Keuls two-way analysis of variance (ANOVA), *p < 0.01, **p < 0.001, ***p < 0.0001, ****p < 0.00001.