Human Scrub Typhus IgM ELISA kit DEIABL75 from Creative Diagnostics

Product Specs
ItemHuman Scrub Typhus IgM ELISA kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIABL75
Quantity96T
Sample Typeserum
ApplicationsELISA
ReactivityHuman
ComponentsThe Scrub Typhus Detect ELISA system for IgM (1 x 96 Wells) contains sufficient reagents for 96 wells.
Each kit contains the following reagents:
Warning:
_ Do not use any reagents where damage to the packaging has occurred.
_ Controls(*) must be centrifuged for 10 seconds at high speed prior to opening the vial to avoid loss of contents.

. 1. Scrub Typhus ELISA Plate:
One strip holder in ziplock foil, containing 96 polystyrene microtiter wells coated with OT derived recombinant antigens in each well. Stable at 2-8°C until the expiration date.
2. Sample Dilution Buffer for Scrub Typhus:
Two bottles, 25 mL each, to be used for preparing sample dilutions. A slight precipitate may form. Mix gently before use. Stable at 2-8°C until the expiration date.
3. Scrub Typhus IgM Positive Control:
One vial, 50 µL. The positive control will aid in monitoring the integrity of the kit as well. Stable at 2-8°C until the expiration date. Before use, quickly centrifuge the vial so that contents can be collected at the bottom.
4. Scrub Typhus Negative Control:
One vial, 50 µL. The negative control will aid in monitoring the integrity of the kit. This is stable at 2-8°C until the expiration date. Before use, quickly centrifuge the vial so that contents can be collected at the bottom.
5. Ready to Use Enzyme Conjugate-HRP for Scrub Typhus IgM:
One bottle, 12 mL of a pre-diluted conjugate to be used as is in the procedure below. Stable at 2-8°C until the expiration date.
Note : The conjugate should be kept in a light-protected bottle at all times as provided.
6. 10X Wash Buffer:
One bottle, 120 mL of 10X concentrate Wash Buffer to be diluted and used in all the washing steps of this procedure. Stable at 2-8°C until the expiration date.
Note : See Preparation of Reagents in Test Procedure section to prepare 1X Wash Buffer.
7. Wash Solution:
One bottle, 20 mL of Wash Solution to be used in between the washing steps after enzyme conjugate-HRP and before liquid TMB addition of this procedure. Stable at 2-8°C until the expiration date.
8. Liquid TMB Substrate:
One bottle, 12 mL of liquid substrate to be used in this procedure. Stable at 2-8°C until the expiration date.
NOTE: The substrate should be kept in a light -protected bottle at all times.
9. Stop Solution:
One bottle, 6 mL to be used to stop the reaction. Stable at 2-8°C until the expiration date.
Caution: strong acid, wear protective gloves, lab coat and safety goggles. Dispose of all materials according to safety rules and regulations.
NOTE: All reagents and controls must be allowed to reach room temperature (20°C~25°C) and mixed thoroughly by gentle inversion prior to use

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Creative Diagnostics

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

Company Profile

Website: https://www.creative-diagnostics.com/

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Human Scrub Typhus IgM ELISA kit from Creative Diagnostics

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Human Scrub Typhus IgM ELISA kit from Creative Diagnostics

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