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Human Scrub Typhus IgM ELISA kit
Human Scrub Typhus IgM ELISA kit from Creative Diagnostics
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Creative Diagnostics for
Human Scrub Typhus IgM ELISA kit
Description
Qualitative
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Product Specs
Item
Human Scrub Typhus IgM ELISA kit
Company
Creative Diagnostics
Price
Supplier Page
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Catalog Number
DEIABL75
Quantity
96T
Sample Type
serum
Applications
ELISA
Reactivity
Human
Components
The Scrub Typhus Detect ELISA system for IgM (1 x 96 Wells) contains sufficient reagents for 96 wells.
Each kit contains the following reagents:
Warning:
_ Do not use any reagents where damage to the packaging has occurred.
_ Controls(*) must be centrifuged for 10 seconds at high speed prior to opening the vial to avoid loss of contents.
. 1. Scrub Typhus ELISA Plate:
One strip holder in ziplock foil, containing 96 polystyrene microtiter wells coated with OT derived recombinant antigens in each well. Stable at 2-8°C until the expiration date.
2. Sample Dilution Buffer for Scrub Typhus:
Two bottles, 25 mL each, to be used for preparing sample dilutions. A slight precipitate may form. Mix gently before use. Stable at 2-8°C until the expiration date.
3. Scrub Typhus IgM Positive Control:
One vial, 50 µL. The positive control will aid in monitoring the integrity of the kit as well. Stable at 2-8°C until the expiration date. Before use, quickly centrifuge the vial so that contents can be collected at the bottom.
4. Scrub Typhus Negative Control:
One vial, 50 µL. The negative control will aid in monitoring the integrity of the kit. This is stable at 2-8°C until the expiration date. Before use, quickly centrifuge the vial so that contents can be collected at the bottom.
5. Ready to Use Enzyme Conjugate-HRP for Scrub Typhus IgM:
One bottle, 12 mL of a pre-diluted conjugate to be used as is in the procedure below. Stable at 2-8°C until the expiration date.
Note : The conjugate should be kept in a light-protected bottle at all times as provided.
6. 10X Wash Buffer:
One bottle, 120 mL of 10X concentrate Wash Buffer to be diluted and used in all the washing steps of this procedure. Stable at 2-8°C until the expiration date.
Note : See Preparation of Reagents in Test Procedure section to prepare 1X Wash Buffer.
7. Wash Solution:
One bottle, 20 mL of Wash Solution to be used in between the washing steps after enzyme conjugate-HRP and before liquid TMB addition of this procedure. Stable at 2-8°C until the expiration date.
8. Liquid TMB Substrate:
One bottle, 12 mL of liquid substrate to be used in this procedure. Stable at 2-8°C until the expiration date.
NOTE: The substrate should be kept in a light -protected bottle at all times.
9. Stop Solution:
One bottle, 6 mL to be used to stop the reaction. Stable at 2-8°C until the expiration date.
Caution: strong acid, wear protective gloves, lab coat and safety goggles. Dispose of all materials according to safety rules and regulations.
NOTE: All reagents and controls must be allowed to reach room temperature (20°C~25°C) and mixed thoroughly by gentle inversion prior to use
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Creative Diagnostics
Phone
: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)
Email: info@creative-diagnostics.com
Company Profile
Website:
https://www.creative-diagnostics.com/
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Human Scrub Typhus IgM ELISA kit from Creative Diagnostics
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Product Specs
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