Fig 2: RAB11FIP5 Transcript in Total PBMC Correlates with Receptor Expression on Total NK Cells, Related to Figure 3(A–F) Spearman correlation of RAB11FIP5 mRNA levels in total PBMC with total NK cells expression of (A) NKG2A, (B) CD85j, (C) iKIR (cocktail of antibodies against KIR2DL1/S5, KIR2DL2/L3/S2, KIR3DL2, KIR3DL1), (D) Siglec 7, (E) FceRI-?, and (F) PLZF. *p < 0.05, **p < 0.01, ***p < 0.001. P values corrected for MCA.(G) Plasma antibody binding to HCMV cell lysate antigen or recombinant HCMV gB antigen measured by ELISA and Bio-Rad clinical CMV assay for individuals in the bnAb (blue) and control group (red). Values displayed as log area under the curve. Cutoff for positivity for the IgG and IgM clinical assay was > 1.1.

Fig 3: Functional Analysis of CD56dim and CD56neg NK Subsets and Gating Strategy for Analysis of NK:Tfh:B Cell Co-culture, Related to Figures 4 and 5(A) Spearman correlation of percentage of total NK cells undergoing degranulation (assessed by CD107a expression) on exposure to target cells with plasma HIV-1 neutralization breadth (PC1).(B and C) Summary boxplots for CD107a expression, IFN-? and TNF-a production from (B) CD56dim NK cells and (C) CD56neg NK cells following target cell stimulation in bnAb (red), control (blue) and HIV seronegative individuals (black). Each symbol represents data from an individual subject and the box-and-whisker plots show the median, quartiles and range. *p < 0.05, **p < 0.01. P values corrected for MCA and viral load.(D) Class-switched memory B cells were identified as live CD3-CD4-CD56-CD19+IgD-IgM-CD20+CD38+/- cells and plasmablasts as live CD3-CD4-CD56-CD19+IgD-IgM-CD20-CD38+ cells. Tfh cells were identified as live CD3+CD4+CD19-CD56- cells. The division index was calculated using Flowjo software. CountBright absolute counting beads were used to calculate absolute numbers. The staining shown is from a representative subject (Tfh+B cell only condition).