rtStar™ tRF&tiRNA Pretreatment Kit AS-FS-005 from Arraystar, Inc

Product Specs
ItemrtStar™ tRF&tiRNA Pretreatment Kit
CompanyArraystar, Inc
Price $205.00Supplier Page View Company Product Page
Catalog NumberAS-FS-005
ApplicationsPCR
TypetRF&tiRNA
Quantity12RXNs

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Arraystar, Inc

9430 Key West Avenue

Suite 128

Rockville, Maryland 20850

United States

Phone: 888-416-6343
240-314-0388
Fax: 240-238-9860

Company Profile

Website: http://www.arraystar.com

(1) Extracellular Vesicle‐Delivered tRF‐His‐GTG‐1 Reprograms Neutrophil Lipophagy and Triggers Inflammation in COVID‐19Advanced ScienceJanuary 14, 2026Tsai‐Ling Liao, Po‐Yu Liu, Yi‐Ming Chen, Kuo‐Tung Tang, Hung‐Jen Liu, Der‐Yuan ChentsRNAs Total tsRNAs were extracted from EVs using the rtStar tRF&tiRNA Pretreatment Kit (Arraystar, AS‐FS‐005) to remove interfering RNA modifications. Synthetic Caenorhabditis elegans miRNA (cel‐miR‐39; 25 fmol;

(2) Peripheral blood tRNA-derived fragments as novel noninvasive biomarkers for diagnosis and prognostic stratification in multiple myelomaFront ImmunolDecember 3, 2025Biyun Yi, Yan Gao, Yanzhao Huang, Kaiyun Guo, Rong Liu, Mengying Zeng, Han Xu, Ming LeiND-1000 instrument. The extracted RNA was pretreated with the rtStar™ tRF & tiRNA Pretreatment Kit (Cat# AS-FS-005, Arraystar) and reverse transcribed into cDNA using the rtStar™ First-Strand cDNA Synthesis Kit (3’

(3) Sperm tRNA-derived fragments expression is potentially linked to abstinence-related improvement of sperm qualityAsian Journal of AndrologySeptember 1, 2025Xi-Ren Ji, Rui-Jun Wang, Zeng-Hui Huang, Hui-Lan Wu, Xiu-Hai Huang, Hao Bo, Ge Lin, Wen-Bing Zhu, Chuan Huangthat could interfere with small RNA-seq library construction using the rtStar™ tRF Pretreatment Kit (AS-FS-005; Arraystar, Rockville, MD, USA). Pretreated RNA was then used for tRF-seq library preparation using

(4) Tumor-derived exosomal tsRNA 3′tiRNA-AlaCGC in promoting fibroblast senescence and Galectin-9 secretion to induce immune tolerance in lung adenocarcinomaCell Death DiscovAugust 25, 2025Guangyin Zhao, Yuchen Zhang, Hongyu Zhang, Yifan Guo, Chang Xu, Di Ge, Jie Gufrom cells and tissues using the Trizol reagent (Life Technologies, USA). RNA Pretreatment Kit (#: AS-FS-005, Arraystar, USA) and rtStar™ First-Strand cDNA Synthesis Kit (#: AS-FS-003, Arraystar, USA) were used

(5) 5′-tiRNA-Lys maintains intestinal epithelial homeostasis by EWSR1-dependent suppression of miR-125a and autophagy activationActa Biochimica et Biophysica SinicaJune 23, 2025Ningqin Xu, Ju Huang, Yiming Wang, Yaxin Liu, Yangwei Jiang, Wei Zhou, Jinghao Sheng, Lahong Zhangconducted to facilitate adapter ligation and reverse transcription by rtStar™ tRF&tiRNA Pretreatment kit (#AS-FS-005; Arraystar, Rockville, USA). Library preparation was performed using the NEBNext Small RNA Library Prep

(6) tRF-Val-TAC-004 protects against renal ischemia-reperfusion injury via attenuating Apaf1-mediated apoptosisiScienceMarch 21, 2025Ni Yin, Xian Xie, Dan Li, Shikun Yang, Yan Liu, Yongzhong Tang, Hao Zhang, Wei ZhangTransZol UP RNA Extraction Kit. tRFs were pretreated by rtStar™ tRF & tiRNA Pretreatment Kit (Arraystar, AS-FS-005, USA) according to the manufacturer’s instruction. The miRNA 1st-strand cDNA Synthesis Kit (Vazyme,

(7) Protocol for detection of tRNA-derived fragments in cells, tissues, and plasmaSTAR ProtocolsDecember 20, 2024Mengqian Yu, Pengyuan Liu, Yan Luassays,Critical commercial assays,Critical commercial assays ,, rtStar tRF&tiRNA pretreatment kit,Arraystar,Cat#AS-FS-005 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Human:

(8) Intravenous injection of BMSCs modulate tsRNA expression and ameliorate lung remodeling in COPD miceStem Cell Research & TherapyNovember 25, 2024Ting Jin, Xianyang Liu, Guoan Li, Shenghua Sun, Lihua Xietotal RNA was subjected to pretreatment using the rtStar™ TRF&tiRNA pretreatment kit (Arraystar Cat# AS-FS-005, USA) and then sequencing libraries were prepared utilized the rtStar™ FirstStrand cDNA synthesis kit

(9) Dynamic PAH-Related Changes: A Dataset of tRNA-Derived Small RNA Transcriptome Across Multiple OrgansScientific DataNovember 20, 2024Yusi Chen, Yi Tang, Tengteng Zhu, Fang Li, Jun Luo, Jingyuan Chen, Yingjie Tan, Tianyu Wang, Jianqiang Peng, Gang Jiang, et al.RNA modifications in tsRNAs, RNA samples were processed with the rtStar tsRNA Pretreatment Kit (Cat# AS-FS-005, Arraystar) to remove modifications, such as acetylation and methylation, that could obstruct small

(10) Distinct fingerprints of tRNA-derived small non-coding RNA in animal models of neurodegenerationDisease Models & MechanismsNovember 1, 2024Sharada Baindoor, Hesham A. Y. Gibriel, Morten T. Venø, Junyi Su, Elena Perez Morrissey, Elisabeth Jirström, Ina Woods, Aidan Kenny, Mariana Alves, Luise Halang, et al.expression. All samples were prepped for the PCR array using a rtStar™ tRF&tiRNA Pretreatment Kit (AS-FS-005, Arraystar) and rtStar™ First-Strand cDNA Synthesis Kit (AS-FS-003, Arraystar). We utilized 1 µg RNA

(11) tsRNA-00764 Regulates Estrogen and Progesterone Synthesis and Lipid Deposition by Targeting PPAR-γ in Duck Granulosa CellsInternational Journal of Molecular SciencesOctober 19, 2024Yaru Chen, Yan Wu, Jinsong Pi, Ming Fu, Jie Shen, Hao Zhang, Jinping Dumodifications and constructed the small RNA-seq libraries with the rtStar™ tRF and tiRNA Pretreatment Kit (AS-FS-005, Arraystar, Rockville, MD, USA). Then, tsRNA sequencing and expression quantification were, respectively,

(12) Dysregulation of pseudouridylation in small RNAs contributes to papillary thyroid carcinoma metastasisCancer Cell InternationalOctober 14, 2024Xi Wang, Hengyuan Gao, Wenjun Pu, Zhipeng Zeng, Nan Xu, Xunpeng Luo, Donge Tang, Yong Daicase of tsRNA, the RNA underwent pretreatment using the rtStar™ tRF&tiRNA Pretreatment Kit (Arraystar, AS-FS-005) to undergo acylation, 3’-cP removal, 5’-P addition, and demethylation. The rtStar™ First-Strand cDNA Synthesis Kit (3’ and 5’ adaptor

(13) Unravelling tRNA fragments in DENV pathogenesis: Insights from RNA sequencingScientific ReportsAugust 7, 2024Deeksha Madhry, Kiran Kumari, Varsha Meena, Riya Roy, Bhupendra Vermasequencing Total RNA was given pre-treatment using the rtStar™ tRF and tiRNA Pretreatment Kit (catalog no. AS-FS-005, Arraystar, MD, USA) to remove internal methylations and terminal modifications for efficient adapter

(14) Threonine fuels glioblastoma through YRDC-mediated codon-biased translational reprogrammingNature CancerMarch 22, 2024Xujia Wu, Huairui Yuan, Qiulian Wu, Yixin Gao, Tingting Duan, Kailin Yang, Tengfei Huang, Shuai Wang, Fanen Yuan, Derrick Lee, et al.TBE–urea gels and then subjected to m1A and m3C demethylation using the rtStar tRF&tiRNA Pretreatment Kit (AS-FS-005, Arraystar). Demethylated tRNA was purified and then partially hydrolyzed according to the Hydro-tRNAseq

(15) Exploring the functional role of tRF-39-8HM2OSRNLNKSEKH9 in hepatocellular carcinomaHeliyonMarch 15, 2024Tianxin Xu, Jie Yuan, Fei Song, Nannan Zhang, Cheng Gao, Zhong Cheninterfere with small RNA-seq library construction or RT-PCR, the rtStar™ tRF&tiRNA Pretreatment Kit (#: AS–FS–005, Arraystar, MD, USA) was used to remove 3′-aminoacyl, 3′-cP, phosphorylate 5′-OH, and demethylate m1A,

(16) Transfer RNA derived fragment, tRF-Glu-CTC, aggravates the development of neovascular age-related macular degenerationTheranosticsJanuary 27, 2024Yu Liang, Lingjie Kong, Yuelu Zhang, Yihan Zhang, Mingsu Shi, Jiaqiu Huang, Hongyu Kong, Siyi Qi, Yunlong Yang, Jiaxu Hong, et al.miRNeasy Mini Kit (Qiagen, 217048). For tsRNA quantification, the RNA pretreatment kit (Arraystar, USA, AS-FS-005) and rtStar First-strand cDNA Synthesis kit (Arraystar, AS-FS-003-02) were used to create cDNA libraries.

(17) Transfer RNA-derived small RNA tRF-Glu-CTC attenuates neointimal formation via inhibition of fibromodulinCellular & Molecular Biology LettersJanuary 3, 2024Qi-Lan Jiang, Jia-Ying Xu, Qing-Ping Yao, Rui Jiang, Qin Xu, Bo-Tao Zhang, Tao Li, Jun Jiangremoved for efficient reverse transcription using the rtStar™ tRF&tiRNA Pretreatment Kit (Catalog# AS-FS -005, Arraystar, MD, USA) [17]. Total RNA from the sample was then ligated to 3′ and 5′ adaptors, and cDNA

(18) tRNA-derived small RNAs are embedded in the gene regulatory network instructing Drosophila metamorphosisGenome ResearchDecember 27, 2023Junling Shi, Jiaqi Xu, Jun Ma, Feng Hebefore reverse transcription, RNAs were pretreated by rtStar™ tRF&tiRNA Pretreatment Kit (Arraystar AS-FS-005) to remove internal m1A, m1G, and m3C. Reverse transcription was performed using HiScript III first Strand cDNA Synthesis Kit

(19) XPOT Disruption Suppresses TNBC Growth through Inhibition of Specific tRNA Nuclear Exportation and TTC19 Expression to Induce Cytokinesis FailureInternational Journal of Biological SciencesOctober 24, 2023Huijuan Dai, Xiaomei Yang, Xiaonan Sheng, Yaohui Wang, Shan Zhang, Xueli Zhang, Lipeng Hu, Zhigang Zhang, Xinrui Dong, Wenjin Yin, et al.size window of 60-100 nt for tRNA. The “demethylation” section in rtStarTM tRF&tiRNA Pretreatment Kit (AS-FS-005; Arraystar) was used for tRNA m1A&m3C demethylation treatment. A 50-µL demethylation reaction mixture

(20) Expression profiles and function prediction of tRNA-derived fragments in gliomaBMC CancerOctober 20, 2023Deng Wei, Ben Niu, Bei Zhai, Xiao-bai Liu, Yi-long Yao, Chan-chan Liang, Ping Wangmanufacturer instructions. Some RNA modifications were removed using rtStarTM tRF&tiRNA Pretreatment Kit (Cat# AS-FS-005, Arraystar). Using 2×miRNA P-RT Solution mix (Shanghai Sangon Biotech, China) and miRNA P-RT Enzyme

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rtStar™ tRF&tiRNA Pretreatment Kit from Arraystar, Inc

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rtStar™ tRF&tiRNA Pretreatment Kit from Arraystar, Inc

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