Human Complement H - CFH ELISA Kit KOA0843 from Rockland Immunochemicals, Inc.

Description

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternative complement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events

Product Specs
ItemHuman Complement H - CFH ELISA Kit
CompanyRockland Immunochemicals, Inc.
Price $715.00Supplier Page View Company Product Page
Catalog NumberKOA0843
Quantity1 Kit
ApplicationsELISA
Conjugate/Tag/LabelUnconjugated
ReactivityHuman
Gene NameCFH
ImmunogenExpression system for standard: NSO; Immunogen sequence: S860-R1231
NCBI Gene AliasesARMD4, HF1, HF2, AMBP1, ARMS1, FHL1, HUS, FH, AHUS1, HF, CFHL3
AnticoagulantHeparin Sodium
TypeELISA Kit
StorageStore vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing
Concentration1 Kit
Detection Range1.56 ng/ml - 100 ng/ml
Sensitivity<50 pg/ml

Specifications/Features

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 100ng/ml, 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml human CFH standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human CFH standard solution and each sample be measured in duplicate.

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