Acetylcholine Receptor Ab ELISA Kit DEIABL27 from Creative Diagnostics

Product Specs
ItemAcetylcholine Receptor Ab ELISA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIABL27
Quantity96T
Sample Typeserum
ApplicationsELISA
ReactivityHuman
ComponentsA. AChR MAb1 Coated Wells, 12 breakapart strips of 8 wells (96 intotal) in a frame and sealed in a foil bag. Allow foilbag to stand at room temperature (20-25°C) for 30 minutes before opening.
Ensure wells are firmly fitted in the frame provided. After opening return any unused wells to the original foil bag and seal with adhesive tape. Then place foil bag in the self-seal plastic bag with desiccant provided, and store at 2-8°C for up to kit expiry date.

B. Foetal Type AChR, 3 vials, Lyophilised
Reconstitute each vial with 0.7 mL reconstitution buffer for AChR (D). Mix gently, and leave to stand at room temperature (20-25°C) for 5 minutes before use. Pool the vials when more than one vial is required, and then use immediately to reconstitute adult type AChR.

C. Adult Type AChR, 3 vials, Lyophilised

B+C :
Reconstitute each vial of C with 0.5 mL of reconstituted foetal type AChR (B) to give a mixture of foetal and adult AChR (B+C). Mix gently, and leave to stand at room temperature (20-25°C) for 5 minutes before use. Pool the vials when more than one vial is required. Use up to 6 hours after reconstitution if stored at 2-8°C.*

D. Reconstitution Buffer for AChR, 5 mL, Ready for use

E. AChR MAb - Biotin (MAb2+MAb3), 3 vials, Lyophilised
Reconstitute each vial with the volume of reconstitution buffer for MAb-Biotin (F) shown on the vial label. Mix gently, and leave to stand at room temperature (20-25°C) for 5 minutes before use. Pool the vials when more than one vial is required. Store at 2-8°C for up to kit expiry date after reconstitution.

F. Reconstitution Buffer for MAb - Biotin, 15 mL, Ready for use

G. Streptavidin Peroxidase (SA - POD), 0.7 mL, Concentrated
Dilute 1 in 20 with diluent for SA-POD (H). For example, 0.5 mL (G) + 9.5 mL (H). Store at 2-8°C for up to kit expiry date after dilution.

H. Diluent for SA - POD, 15 mL, Ready for use

J. Peroxidase Substrate (TMB), 15 mL, Ready for use

K. Stop Solution, 10 mL, Ready for use

L. Concentrated Wash Solution, 100 mL, Concentrated
Dilute 10 X with pure water before use. For example, 100 mL (L) + 900 mL pure water. Use up to kit expiry date after dilution.

M1-4. Calibrators, 0.5, 1.0, 6.5 and 20 nmol/L toxin bound, 4 x 0.7 mL, Ready for use

N. Negative Control, 3 mL, Ready for use

P1-2. Positive Controls l & ll (see label for concentration range), 2 x 0.7 mL, Ready for use

* The absorbance at 450 nm will be 10-15% lower when reconstituted receptors have been stored for 6 hours at 2-8°C
Detection TargetAChR

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Creative Diagnostics

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

Company Profile

Website: https://www.creative-diagnostics.com/

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Acetylcholine Receptor Ab ELISA Kit from Creative Diagnostics

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Acetylcholine Receptor Ab ELISA Kit from Creative Diagnostics

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