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Human Endothelial lipase ELISA Kit
Human Endothelial lipase ELISA Kit from Creative Diagnostics
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Creative Diagnostics for
Human Endothelial lipase ELISA Kit
Description
Quantitative
Product Specs
Company Info
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Product Specs
Item
Human Endothelial lipase ELISA Kit
Company
Creative Diagnostics
Price
Supplier Page
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Catalog Number
DEIABL60
Quantity
96T
Detection Range
0.625 ng/ml-40 ng/ml
Sample Type
serum, plasma, cell culture supernates, urine, tissue homogenates
Applications
ELISA
Sensitivity
The minimum detectable dose of human EL is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations
Components
1. Assay plate (12 x 8 coated Microwells): 1(96 wells)
Opened kit: May be stored for up to 1 month at 2 - 8°C. Try to keep it in a sealed aluminum foil bag, and avoid the damp.
2. Standard (Freeze dried): 2
3. Biotin-antibody (100 x concentrate): 1 x 120 µl
4. HRP-avidin (100 x concentrate): 1 x 120 µl
Opened kit: May be stored for up to 1 month at 2 - 8°C. If don't make recent use, better keep it store at -20°C.
5. Biotin-antibody Diluent: 1 x 15 ml
6. HRP-avidin Diluent: 1 x 15 ml
7. Sample Diluent: 1 x 50 ml
8. Wash Buffer (25 x concentrate): 1 x 20 ml
9. TMB Substrate: 1 x 10 ml
10. Stop Solution: 1 x 10 ml
Opened kit: May be stored for up to 1 month at 2 - 8°C.
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11. Adhesive Strip (For 96 wells): 4
12. Instruction manual: 1
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess
Reactivity
Human
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Creative Diagnostics
Phone
: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)
Email: info@creative-diagnostics.com
Company Profile
Website:
https://www.creative-diagnostics.com/
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Human Endothelial lipase ELISA Kit from Creative Diagnostics
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Product Specs
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