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Salivary Melatonin ELISA Kit
Salivary Melatonin ELISA Kit from Creative Diagnostics
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Creative Diagnostics for
Salivary Melatonin ELISA Kit
Description
Quantitative
Product Specs
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Product Specs
Item
Salivary Melatonin ELISA Kit
Company
Creative Diagnostics
Price
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Catalog Number
DEIA-S10024
Quantity
96T
Applications
ELISA
Sample Type
saliva
Sensitivity
The lower limit of sensitivity was determined by interpolating the mean optical density minus 2 SDs of 10 sets of duplicates randomized across the plate within one run at the 0 pg/mL level. The minimal concentration of Melatonin that can be distinguished from 0 is 0.58 pg/mL.
Functional Sensitivity: The functional sensitivity was determined as the concentration of 11 saliva samples, tested as 20 replicates each, resulting in a CV of 30% or less. The functional sensitivity of the Salivary Melatonin ELSIA is 1.9 pg/mL
Reactivity
Human
Detection Target
Melatonin
Components
1. Microtitre Plate Coated with rabbit antimelatonin monoclonal antibodies. 1/96-well.
2. Melatonin Standard in a Trizma buffered solution with stabilizer protein and a nonmercury preservative. Serially dilute before use according to Reagent Preparation. Higher order melatonin CRM: 50 pg/mL. 1 vial, 1.5 mL.
3. Melatonin Controls. High, Low, in a Trizma buffered solution with stabilizer protein and a non-mercury preservative. Refer to control insert for ranges. 2 vials, 1 mL each.
4. Wash Buffer Concentrate (10X). Dilute before use according to Reagent Preparation. Contains: phosphate buffer, detergent, preservative. 1 bottle/ 100 mL.
5. Melatonin Enzyme Conjugate Concentrate. Dilute before use with Melatonin Assay Diluent (see step 5 of Procedure). Contains: melatonin conjugated to HRP, buffer, preservative. 1 vial/ 75 µL.
6. Melatonin Assay Diluent. Ready to use. Contains a Trizma buffer with protein stabilizer and preservative. 1 bottle/ 30 mL.
7. TMB Substrate Solution Non-toxic, ready to use. 1 bottle/25 mL.
8. 2 M Stop Solution Contains: sulfuric acid. 1 bottle/ 12.5 mL
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Creative Diagnostics
Phone
: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)
Email: info@creative-diagnostics.com
Company Profile
Website:
https://www.creative-diagnostics.com/
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Salivary Melatonin ELISA Kit from Creative Diagnostics
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Product Specs
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