Total human IgE ELISA Kit DEIABL50 from Creative Diagnostics

Product Specs
ItemTotal human IgE ELISA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIABL50
Quantity96T
Detection Range3.125 ng/ml - 200 ng/ml
Sample TypePlasma, Serum
Sensitivity0.912 ng/ml
TemperatureThe expiration date for the package is stated on the box label.
1. DILUENT
The 5X Diluent Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8°C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16-25°C) or at 4-8°C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-IgE conjugate should be stored at 4-8°C and diluted immediately prior to use. The working conjugate solution is stable for up to 1 hour when stored in the dark.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8°C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8°C and is stable until the expiration date.
6. ANTI-HUMAN IgE ELISA MICRO PLATE
Anti-Human IgE coated wells are stable until the expiration date, and should be stored at 4-8°C in sealed foil pouch with desiccant pack.
7. HUMAN IgE CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4°C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
INDICATIONS OF INSTABILITY
If the test is performing correctly, the results observed with the standard solutions should be within 20 % of the expected values
ApplicationsELISA
ReactivityHuman
Components1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 mL of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 mL of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 µL of affinity purified anti-Human IgE antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 mL 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN IgE ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human IgE.
7. HUMAN IgE CALIBRATOR
One vial containing of Human IgE calibrator

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Creative Diagnostics

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

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Website: https://www.creative-diagnostics.com/

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Total human IgE ELISA Kit from Creative Diagnostics

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Total human IgE ELISA Kit from Creative Diagnostics

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