Proteome Profiler Human XL Cytokine Array Kit from R&D Systems, a Bio-Techne Brand

Product Specs
ItemProteome Profiler Human XL Cytokine Array Kit
CompanyR&D Systems, a Bio-Techne Brand
Price Supplier Page View Company Product Page
Catalog NumberARY022B
ReactivityHuman
Quantity1 Kit
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Namedickkopf WNT signaling pathway inhibitor 1
NCBI Gene AliasesDKK-1, SK

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) ATP13A2 Loss of Function-Driven Polyamine Dysregulation Induces SAM Depletion and Epigenetic Astrocyte ToxicitybioRxivApril 6, 2026Elena Coccia, Gustavo Morrone Parfitt, Sundas Ijaz, Aisha Sati, Julia Gesner, Andrea Perez Arevalo, Jennifer Strong, Anna Bright, Soha Sohail, Thea Meimoun, et al.conditioned media were analyzed using the Proteome Profiler™ Human XL Cytokine Array Kit (R&D Systems, #ARY022B) according to the manufacturer’s instructions. Following the assay, the membranes were developed using

(2) LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasisiScienceMarch 20, 2026Ke-En Tan, Zuag Paj Her, Cheng Zhang, Kok Siong Yeo, Taylor Weiskittel, Cassie Howe, Shyang Hong Tan, Katherine M. Johnson, Gina L. Razidlo, Thomas E. Rubino, et al.LMO1-overexpressing BE2C cells using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, #ARY022B) according to the manufacturer’s instructions. Signal intensities for the cytokine array blots were

(3) Pro-Tumorigenic Signaling Between Small Extracellular Vesicles of Cancer Cells and Bone Marrow-Derived Mesenchymal Stem Cells-An In Vitro StudyInt J Mol SciMarch 13, 2026Jyothi Attem, Ram Mukka Raju Jogula, Swathi Kaliki, Geeta K. Vemuganticytokine/chemokine dot blot analysis. Protein expression was measured following the manufacturer’s protocol (ARY022B, R&D Systems, Minneapolis, MN, USA). Briefly, for blocking, 2 μL of Array Buffer 6 was added to the

(4) An interfacial hydrogel compartment within a multicompartment tendon-to-bone scaffold influences cell behavior under cyclic tensile loadingRegenerative BiomaterialsMarch 9, 2026Kyle B Timmer, Megan L Killian, Brendan A C Harleyarray. Secreted protein release was measured via a Proteome Profiler Human XL Cytokine Array Kit (#ARY022B, R&D Systems), run according to manufacturer recommendation using previously described methods [54].

(5) A small‑molecule HDAC/PDE modulator activates human adipocyte UCP1 and resolves inflammatory signalingJ Transl MedMarch 5, 2026Mayyasa Rammah, Atilio Reyes Romero, Najeeb Halabi, Sirin Abuaqel, Andrea Guennoun, Alexander Dömling, Jingxuan Shan, Lotfi Chouchaneprofiles were analyzed using the Proteome Profiler Array, Human XL Cytokine Array Kit (R&D Systems, ARY022B) following the manufacturer’s protocol. Briefly, cell culture supernatants were diluted and incubated

(6) Senescence-directed nanotherapy ameliorates fibrosis and overcomes immune exclusion in cancerbioRxivMarch 4, 2026Clemens Hinterleitner, Valentin J. A. Barthet, Hailey V. Goldberg, Kristen C. Vogt, Ana Marie Perea, Stephen Ruiz, Logan R. Hillger, Domhnall McHugh, Yu-Jui Ho, Almudena Chaves-Perez, et al.at −80 °C until use. Assays were using the Proteome Profiler Mouse XL Cytokine Array (R&D Systems, ARY022B) and the BioRad Imager (ChemiDoc MP Imaging System) with automated optimal exposure following the manufacturer’s

(7) A Novel Competing Endogenous RNA Linked to Dysregulated Neuroinflammation in Alzheimer’s DiseaseCellsFebruary 27, 2026Dinesh Devadoss, Juliet Akkaoui, Natalia Orso, Thiruselvam Viswanathan, Glen M. Borchert, Madepalli K. Lakshmana, Hitendra S. Chandremodeling factors were analyzed using the Proteome Profiler Human XL (hXL) Cytokine Array Kit (cat-# ARY022B; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. Equal protein amounts

(8) Cutaneous diffuse large B-cell lymphoma induces a macrophage immunosuppressive phenotype through IL-10 secretion∗Blood AdvancesFebruary 10, 2026Jean Galtier, Rémi Peanne, Valérie Le Morvan, Thibaut Blondy, Maïlys Melane, Aurore Bidon, Arnaud Chopin, Martina Prochazkova-Carlotti, Sandrine Poglio, Jean-Philippe Merlio, et al.(Biolegend). Cytokines secreted by ARSI cells in CM were analyzed using the Proteome Profiler Human XL Cytokine Array (ARY022B; Bio-Techne) according to the manufacturer’s instructions. The signal was visualized on a ChemiDoc imaging system

(9) IL-6R blockade with tocilizumab disrupts pericyte- and tumor cell-driven IL-6/STAT3 signaling, enhancing docetaxel efficacy in ER+ breast cancerbioRxivJanuary 31, 2026Roza Kamila Przanowska, Jacky Gomez-Villa, Victoria J. Liu, Natalia Antonides-Jensen, Lavanya Visvabharathy, John C. Alverdy, Sonia L. Hernandez, Samantha S. Yeemeasured using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, part of Bio-Techne #ARY022B). Conditioned media were either used fresh, right away, or stored in 600 μL aliquots in the −80°C freezer.

(10) Non-Canonical Senescence Phenotype in Resistance to CDK4/6 Inhibitors in ER-Positive Breast CancerBiomoleculesJanuary 6, 2026Aynura Mammadova, Yuan Gu, Ling Ruan, Sunil S. Badve, Yesim Gökmen-Polarwas performed using the Proteome Profiler Array Human XL Cytokine Array Kit (Bio-Techne/R&D Systems, #ARY022B, Minneapolis, MN, USA), according to the manufacturer’s instructions. Imaging and quantification were

(11) Lung Microphysiological System Validates Novel Cell Therapy for Acute Respiratory Distress SyndromeAdvanced BiologyJanuary 1, 2026Bokyong Kim, So‐Hui Kim, Jieun Kim, Eun‐Young Eo, Hyung‐Jun Kim, Jae Ho Lee, Choon‐Taek Lee, Taeho Kong, Su Kyoung Seo, Seunghee Lee, et al.analyzed for human cytokine and chemokine profiles using the Human XL Cytokine Proteome Profiler Array (ARY022B, R&D Systems, Minneapolis, USA), a membrane‐based antibody array. The intensity of each spot on the

(12) Semaphorin-7A promotes macrophage-mediated mammary epithelial and ductal carcinoma in situ invasionBreast Cancer ResearchDecember 26, 2025Petra A. Dahms, Brendan Hinckley, Rytis Prekeris, Fariba Behbod, Traci R. Lyonsmedia was measured via the commercially available kit for human cytokines (Bio-techne, R&D Systems, ARY022B) according to manufacturer instructions. Arrays were imaged on a LI-COR Odyssey CLx imager (LI-COR Biosciences,

(13) Identification of senescence-associated drivers of tumour growth and progression using a novel microarray platformbioRxivDecember 26, 2025Hui-Ling Ou, Reuben Hoffmann, Rebecca Smith, Kaylyn Devlin, Mark Dane, Daniel Muñoz-Espín, James E Korkolaany residual cell debris before being subjected to Proteome Profiler Human XL Cytokine Array kit (#ARY022B, R&D Systems). Arrays were visualised with ECL Prime Western Blotting detection reagents (#RPN2232,

(14) SLC6A14-mediated glutamine promotes SYTL4–CXCL8 axis activation to drive gemcitabine resistance and immune evasion in pancreatic cancerExperimental & Molecular MedicineDecember 25, 2025Hyeon Woong Kang, Ju Hyun Kim, Jae Woong Jeong, Sungsoon Fang, Won-Gun Yun, Hye-Sol Jung, Wooil Kwon, Jin-Young Jang, Hyo Jung Kim, Joon Seong Parkcentrifugation and assayed using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, cat. no. ARY022B) according to the manufacturer’s instructions. The supernatants were incubated for 24 h at 4 °C and

(15) Intracellular C3 regulates the immune response to infection via NF-κB signalingCell Mol Life SciDecember 24, 2025Katarzyna Kuska, Serena Bettoni, Frida Mohlin, Maja Chrobak, Vaishnavi Dandavate, Saleh Moradi, Ben King, Kristian Riesbeck, Anna M. Blomlysates were used to detect cytokines using Proteome Profiler Human XL Cytokine Array Kit (R&D systems #ARY022B), Bio-Plex Pro human cytokine 27-plex assay (Bio-Rad #M500KCAF0Y) or commercial ELISAs from R&D systems

(16) Stiffness-mediated paracrine signaling enhances induction of EMT in oral squamous cell carcinomaAPL BioengDecember 23, 2025So Youn Moon, Marcelo Lazzaron Lamers, Adam J. Englerand Cal27 soft-CM—were measured by the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems; ARY022B). 2 × 105 cells of SCC25 on a plastic tissue culture plate, and the same amount of Cal27 were seeded

(17) Beyond cytotoxicity: pollutant mixtures elicit unconventional epithelial-fibroblast signaling in a human lung air-liquid interface co-culture modelFront ToxicolDecember 18, 2025Justine Fredoc-Louison, Maëva Cherrière, Bastien Rival, Suzanne De Araujo, Sabine François, Samir Dekalisemi-quantitative analysis of 105 soluble proteins was performed using the Human XL Cytokine Array Kit (ARY022B, R&D Systems, USA) on pooled basolateral media (n = 3 independents experiments) from hAELVi, MRC-5 and

(18) Palmitic but Not Oleic Acid Induces Pro-Inflammatory Dysfunction of Human Endothelial Cells from Different Vascular Beds In VitroInt J Mol SciDecember 17, 2025Daria Shishkova, Victoria Markova, Yulia Yurieva, Alexey Frolov, Anastasia Lazebnaya, Maxim Sinitsky, Anna Sinitskaya, Vera Matveeva, Evgenia Torgunakova, Alexander Stepanov, et al.in the cell culture supernatant were performed using Proteome Profiler Human XL Cytokine Array Kits (ARY022B, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. In order to improve

(19) Extracellular vesicles derived from pro-inflammatory M1 macrophages induce an inflammatory and invasive phenotype in melanoma cellsCell Communication and SignalingDecember 3, 2025Kaisa Mäki-Mantila, Einari A. Niskanen, Kirsi Kainulainen, Laia Puig Pardas, Niina Aaltonen, Wafa Wahbi, Piia Takabe, Aino Rönkä, Kirsi Rilla, Sanna Pasonen-Seppänen(Revvity). Analysis sequence is shown in Supplementary file 1. Cytokine array Human XL Cytokine array kit (ARY022B, R&D Systems) was used to measure the cytokine content of M0 and M1 EVs and the production of cytokines

(20) Mesenchymal stromal cells modulate neutrophil phenotype via paracrine signalsStem Cell Res TherOctober 21, 2025Laudy Cherry, Sinziana Popescu, Carmen Alexandra Neculachi, Evelyn-Gabriela Nastase-Rusu, Catalina Iolanda Marinescu-Colan, Bogdan Paul Cosman, Letitia Ciortan, Fabio Martelli, Maya Simionescu, Elena Butoi, et al.and after N1/N2 polarization was analysed using the Proteome Profiler Human XL Cytokine Array Kit (ARY022B, R&D Systems) following the manufacturer’s instructions. Briefly, the culture medium was collected after

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(1)

5/13/2020Works Great to Measure Inflammatory Mediators Scott ScarneoThis works great to detect up to 100+ cytokines and chemokines in samples.
(1)

3/11/2019Easy to Use with Fast Results Scott ScarneoEasy and works well.