Description
Intended Uses: For the quantitative detection of Hamster Thioredoxin (TRX) concentration in serum, plasma and other biological fluids.
Principle of the Assay::This assay employs a two-site sandwich ELISA to quantitate TRX in Hamster serum, plasma. An antibody specific for TRX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals.Thioredoxins are characterized at the level of their amino acid sequence by the presence of two vicinal cysteines in a CXXC motif. These two cysteines are the key to the ability of thioredoxin to reduce other proteins. Thioredoxin proteins also have a characteristic tertiary structure termed the thioredoxin fold.The thioredoxins are kept in the reduced state by the flavoenzyme thioredoxin reductase, in a NADPH-dependent reaction. Thioredoxins act as electron donors to peroxidases and ribonucleotide reductase