Description
Background/Introduction: Glutamine Synthetase (GS) is mainly present in plants, is one of the key enzymes in vivo assimilation of ammonia. GS can catalyze ammonium ions and glutamic acid to synthesise glutamine Gln, not only can prevent excessive biological toxic ammonium ions, but also glutamine Gln is the main storage and transport in the form of ammonia. In the presence of ATP and Mg 2+, GS can catalyze ammonium ions and glutamic acid to synthesise glutamine Gln; glutamine Gln further converted to Gamma-glutamyl hydroxamic acid, under acidic conditions to form a red iron complexes; the complex has a maximum absorption peak at 540nm