Description
Background/Introduction: Alkaline protease is a kind of endopeptidase. It is widely used to hydrolyze different proteins to peptides and amino acid. And is widely used in several industries that include leather processing, meat processing, dairy, preparation of organic fertilizer, silk industry, and also for the recovery of silver from used X-ray films. Among all proteases, alkaline proteases are robust in nature, and are primarily used as detergent additives. The assay is initiated with the enzymatic catalysis of casein by AKP in alkaline environment. The enzyme catalysed reaction products can be measured at a colorimetric readout at 680 nm