Description
Background/Introduction: Cytochrome P450 enzymes play an important role in the metabolism of exogenous substrate, especially drugs and poisons. As an important enzyme of P450 family, Aniline 4-Hydroxylase (AH) is equivalent to isoform CYP2E1. CYP2E1 not only participate the drug metabolism, but also catalytic the activation of variety of precarcinogens and prepoison. AH catalytic the hydroxylation of aniline, and further convert to pheno-indole compound, which has a characteristic absorption peak at 630nm; AH activity was calculate by measuring the absorbance change rate at 630nm