Description
Background/Introduction: Acetylcholinesterases (AChEs) are enzymes that hydrolyze the neurotransmitter acetylcholine (ACh) to and choline. AChE is located at the synaptic cleft and functions to terminate synaptic transmission by catalyzing the breakdown of ACh allowing cholinergic neurons to return to a resting state after activation. Changes in AChE activity may result from exposure to certain insecticides, which act as cholinesterase inhibitors. Inhibitors of AChE are also used to treat certain conditions such as dementia. The Acetylcholinesterase Activity Microplate Assay Kit provides a simple and direct procedure for measuring AChE levels in a variety of samples such as blood, serum, and plasma. In this assay, thiocholine produced by AChE, reacts with DTNB to form an colorimetric (412 nm) product (TNB), proportional to the AChE activity present