Description
Intended Uses: For the quantitative detection of Pig Caspase-8 (CASP8) concentration in serum, plasma and other biological fluids.
Principle of the Assay::This assay employs a two-site sandwich ELISA to quantitate CASP8 in Pig serum, plasma. An antibody specific for CASP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CASP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CASP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CASP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Caspase 8 is a member caspase family. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined