Fig 1: Protein expression of the minor tryptophan transport LAT2 normalized to the methodological reference condition regular medium.A 1 h exposure to serosal fluids alone, (B) exposure to 1 h serosal fluids as preventive measure before oxidatively stressing the cells (by 10 µM H2O2), (C) exposure to 1 h serosal fluids as a treatment measure after oxidatively stressing the cells, (D) 24 h exposure to serosal fluids alone, (E) exposure to 24 h serosal fluids as preventive measure before oxidatively stressing the cells, and (F) exposure to 24 h serosal fluids as a treatment measure after oxidatively stressing the cells. X-axis presents exposure to regular medium (RM), serosal fluid carrier solution G-Krebs, and serosal fluid pool derived from healthy biopsies collected from the unexposed colon (HC-CON), from healthy biopsies collected after in vivo butyrate exposure (HC-BUT), from irritable bowel syndrome biopsies collected from the unexposed colon (IBS-CON), from IBS biopsies collected after in vivo butyrate exposure (IBS-BUT), from healthy biopsies ex vivo exposed to supernatant of fecal control fermentation (HC-FERCON), and from healthy biopsies ex vivo exposed to supernatant of fecal fiber fermentation (HC-FERFIB). Per condition n ≤ 5 biologically independent experiments. Data visualized as boxplots, horizontal line indicates median, whiskers indicate minimum to maximum, individual data points are overlayed. Black boxpots present control conditions RM and G-Krebs, green presents HC-CON and HC-BUT, blue presents IBS-CON and IBS-BUT, purple presents HC-FERCON and HC-FERFIB. One-way ANOVA with posthoc uncorrected Fisher’s LSD multiple comparisons test versus G-Krebs control condition. Exact p-value for ANOVA and if significant for posthoc tests provided in figure.
Fig 2: Gene expression of subunit SLC7A8 of the minor tryptophan transport LAT2 normalized to reference gene GAPDH and the methodological reference condition regular medium.A 1 h exposure to serosal fluids alone, (B) 24 h exposure to serosal fluids alone, (C) exposure to 24 h serosal fluids as preventive measure before oxidatively stressing the cells (by 10 µM H2O2), and (D) exposure to 24 h serosal fluids as a treatment measure after oxidatively stressing the cells. X-axis presents exposure to regular medium (RM), serosal fluid carrier solution G-Krebs, and serosal fluid pool derived from healthy biopsies collected from the unexposed colon (HC-CON), from healthy biopsies collected after in vivo butyrate exposure (HC-BUT), from irritable bowel syndrome biopsies collected from the unexposed colon (IBS-CON), from IBS biopsies collected after in vivo butyrate exposure (IBS-BUT), from healthy biopsies ex vivo exposed to supernatant of fecal control fermentation (HC-FERCON), and from healthy biopsies ex vivo exposed to supernatant of fecal fiber fermentation (HC-FERFIB). Per condition n ≤ 5 biologically independent experiments. Data visualized as boxplots, horizontal line indicates median, whiskers indicate minimum to maximum, individual data points are overlayed. Black boxpots present control conditions RM and G-Krebs, green presents HC-CON and HC-BUT, blue presents IBS-CON and IBS-BUT, purple presents HC-FERCON and HC-FERFIB. Commonly used thresholds for biological relevance indicated by the gray dashed lines for 0.5- and 2-fold changes compared to G-Krebs. One-way ANOVA with posthoc uncorrected Fisher’s LSD multiple comparisons test versus G-Krebs control condition. Exact p-value for ANOVA and if significant for posthoc tests provided in figure.
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