Description
Principle of the Assay::The Human C-Reactive Protein ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human C-Reactive Protein in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human C-Reactive Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and C-Reactive Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human C-Reactive Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of C-Reactive Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver