Description
Intended Uses: The CA125 ELISA Kit is intended for the quantitative determination of the Cancer Antigen CA125 concentration in human serum.
Principle of the Assay: The CA125 ELISA test is an adapted solid phase 1-step sandwich ELISA. Samples, a biotinylated mouse anti-CA-125 capture antibody, and mouse anti-CA-125-HRP conjugate are all added to wells coated with streptavidin. CA-125 in the patient sample binds to the biotinylated capture antibody. The biotinylated capture antibody simultaneously binds to the streptavidin coated plate. Anti-CA-125-HRP enzyme conjugate forms a sandwich around captured CA-125. Unbound antibodies are washed off. TMB substrate is added resulting in the development of a blue color. The concentration of CA-125 is directly proportional to the color intensity developed. A standard curve is generated relating color intensity to CA-125 concentration