Description
Background/Introduction: In humans and primates, uric acid is the end product of purine metabolism. It is produced by the oxidation of xanthine and hypoxanthine by xanthine oxidase and excreted in urine. High serum levels of uric acid, hyperuricemia, are associated with insulin resistance, cardiovascular disease, and gout. The mechanisms leading to hyperuricemia are typically either increased uric acid production or decreased urine excretion. Increased serum uric acid may be a marker of renal disease. The assay is initiated with the enzymatic catalysis of the uric acid by uricase. The enzyme catalysed reaction products quinone can be measured at a colorimetric readout at 505 nm