Description
Background/Introduction: Sucrose phosphate synthase (SPS, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue. Fructose-6-phosphate is catalyzed by sucrose phosphate synthase to generate sucrose phosphate, and then react with resorcinol present a color change, have acharacteristic absorption peak at 480nm. The intensity of the product color, measured at 480 nm, is proportionate to the enzyme activity in the sample